2022-2023<\/h3>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>\n\t\n\t\t\n\t\t\tAndre Berndt<\/strong><\/a>, PhD, Assistant Professor, Department of Bioengineering, University of Washington<\/strong><\/p>\nMassively parallel, high throughput engineering of optogenetic biosensors for neuronal signaling<\/em><\/strong><\/p>\nFlorescent, genetically encoded proteins have revolutionized the study of brain cells and neural circuits. By literally lighting up in the presence of specific neural activity, which then can be recorded by microscopes and light fibers in living brains, this tool has unlocked many mysteries and allowed researchers to visualize brain activity and neural pathways. But there has been a bottleneck: Developing and identifying the best sensor for each experiment. These encoded proteins need to react in the presence of only specific stimuli, in some cases might need to be highly sensitive, in other cases may need to fluoresce for a longer period of time, or an experiment may need two sensors to see how multiple neurotransmitters interact.<\/p>\n
In the past, each sensor had to be genetically modified, produced, and tested individually. Perhaps only a few dozen or hundred could be compared, and researchers chose the best option from a small sample \u2013 not knowing if there was a better, more precise option available. Dr. Berndt has developed a process for developing and testing very large numbers of optogenetic biosensors simultaneously, aiming for screening more than 10,000 per day and building a massive library of biosensors that can give researchers access to precisely engineered proteins they can use to run ever-more specific experiments.<\/p>\n
The technology uses rapid genetic engineering to create large numbers of variants of a biosensor, then places individual variants into a microwell array. The sensors are exposed to neuropeptides \u2013 currently, Dr. Berndt is focusing on ligand-specific opioid sensors \u2013 and optical sensors then read the microarray, detecting the brightness and other variables of each variant, and selecting the best options for further testing. Over the course of 2 years, some 750,000 biosensors will be tested and the process for their screening refined, advancing research into opioid actions in the brain and providing a versatile approach other researchers can use for their experiments.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\tRuixuan Gao<\/strong><\/a>, Ph.D., Assistant Professor, Department of Chemistry and Department of Biological Sciences, University of Illinois Chicago<\/strong><\/p>\nSub-10 nm spatial profiling of synaptic proteins and RNA transcripts with high-isotropy expansion microscopy using a highly homogenous hydrogel constructed from tetrahedron-like monomers<\/em><\/strong><\/p>\nTo examine things that are very small \u2013 like the neurons and their synapses in the brain \u2013 researchers use powerful microscopes. But there is another approach that can yield impressive results: literally expanding a tissue sample and the cells within it by use of a special swellable hydrogel through a process called expansion microscopy. The hydrogel binds to different molecular components of cells and expands, ideally holding all the component parts in the same relative position to one another, creating a larger and more accessible sample to study \u2013 in principle, similar to writing on a balloon, then inflating it.<\/p>\n
However, the current hydrogels used for this process have some drawbacks when it comes to studying minute structures in the brain. The margin of error in holding the relative position of molecules isn\u2019t as precise as desired. New gel that potentially overcomes this issue reacts poorly to the heat used in denaturing and treating tissue samples. And it can limit the use of fluorescing biomarkers. Dr. Gao aims to improve the technology by developing a new type of \u201ctetra-gel\u201d, which is chemically engineered to have a tetrahedron-shaped monomer that is extremely uniform as it expands, resists heat and allows the use of bioluminescent markers. He will also develop chemical linkers, specialized molecules that will bind different molecular components of the sample to the gel. The goal is to have an expanded sample that matches the fidelity of the original to within 10 nanometers, matching the resolution of powerful microscopes.<\/p>\n
Dr. Gao\u2019s research has already identified promising compounds with which to develop this tetra-gel. As his lab develops and refines it, he will apply its abilities to the study of, for example, early-onset Parkinson\u2019s Disease affected brains. Studying the exact structure of these brains has been challenging with traditional methods, and the goal is to precisely map synaptic proteins and associated gene transcripts, helping uncover how the early onset PD brain is molecularly structured.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\tMirna Mihovilovic Skanata<\/strong><\/a>, Ph.D., Assistant Professor, Physics Department, Syracuse University <\/strong><\/p>\nTwo-photon tracking technology to read and manipulate neural patterns in freely moving animals <\/em><\/strong><\/p>\nThe gold standard for neuroscientists is to be able to record and manipulate what is happening in the brain at a high level of precision, over a large area, while a living animal is behaving freely and naturally. Over the years, technology has allowed researchers to move towards this ideal, but always with some compromises. Often, animals needed to be head-fixed, and\/or have intrusive sensors or optics implanted in their brains, and often high-fidelity recording or manipulation was limited to a relatively small area of the brain, while broad-based recordings and manipulation was less precise.<\/p>\n
One of the key challenges is simply the motion and distortion of the brain and neurons in a freely-moving animal. But Dr. Skanata is developing a new two-photon tracking technology that allows her to track multiple individual neurons in a moving animal without any invasive implants, and optically activate or manipulate those neurons. The model used is fruit fly larvae, which are naturally transparent, and the system Dr. Skanata will continue to develop uses two-photon microscopes (which allow very precise targeting) coupled with an ingenious algorithm that can rapidly detect the motion of individual neurons and adjust the position of the subject on a moving stage to keep it centered under the microscope. The system calculates the relative positions of multiple neurons, adjusts for the motion and deformation of the brain during movement, and tracks neural activity across a large area.<\/p>\n
When tracking an animal that has been modified so that neurons can be activated when exposed to optical light, the system lets researchers switch on neurons with high precision during natural activity. Importantly, the system Dr. Skanata is developing has the capability to independently control two laser beams, so it can track multiple areas simultaneously, and will even allow tracking activity among individuals, allowing insight into neural activity during group encounters.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>\n\t\n\t\t\n\t\t\t2021-2022<\/h3>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>\n\t\n\t\t\n\t\t\tTimothy Dunn, Ph.D.,<\/a><\/strong> Assistant Professor, Department of Biomedical Engineering, Duke University<\/strong><\/p>\nMulti-scale Three Dimensional Behavioral Quantification in Individuals and Social Groups<\/em><\/strong><\/p>\nCurrent methods of measuring movement of freely behaving animals have limitations: Highly detailed observations of small movements of an animal (a single digit, for example) require restricted ranges of motion. Studying freely moving behavior in 3D space often means limiting resolution, perhaps only tracking overall position, or relying on an observer\u2019s description. Automatic video tracking in animals typically requires an unnatural, simple environment, and body parts not visible to cameras aren\u2019t tracked accurately. High-resolution Artificial Intelligence (AI) predictions over large three-dimensional spaces using volumetric spatial representation, a technique recently developed to overcome these issues, require massive computing power. Adding multiple animals for social observations introduces additional issues.<\/p>\n
As a result, there is poor availability of the most desired data: High-resolution, automatic tracking of animals in 3D space performing natural behaviors, alone or in groups, and quantification of that motion in a standardized format. Dr. Dunn is working on a new approach that aims to bring that ideal closer. Building on learnings from a 3D geometric machine-learning algorithm his team used to greatly improve the accuracy of predictions, Dr. Dunn and his team are now working on adaptive recurrent image sampling (ARIS) that combines images from multiple cameras to build a model that can measure and predict body position on many scales, even when a part (such as an arm or foot) isn\u2019t directly visible.<\/p>\n
ARIS selectively improves the resolution of fine-scale body features, and uses predictive modelling based on what it knows of its subject (arrangement and length of limbs, how they are connect, how they move, etc.) \u2013 learned first by parsing enormous amounts of training data from freely-behaving rats and then fine-tuned using training data in other species \u2013 to focus on the portion of space where the body part is likely to be. This uses far less computational power than previous 3D volumetric tools. In his research, Dr. Dunn will implement ARIS and record data at multiple scales, from overall position and posture down to the movement of fine features of the hands, feet, and face. Further research will explore its effectiveness with multiple animals interacting. This ability to measure behavior in a new, more precise way has broad implications for the study of neurological disorders that affect movement, linking brain activity to behavior, and studying social interactions.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\tJeffrey Kieft, Ph.D.,<\/a><\/strong> Professor, Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine<\/strong><\/p>\nA New Technology to Control the Transcriptome<\/em><\/strong><\/p>\nMessenger RNA, or mRNA, is recognized as a vital player in the life and health of cells. These RNA molecules are the templates to make protein, and are created within cells to carry instructions to the protein-making machinery, then are destroyed by enzymes. The totality of mRNA an organism expresses is called its \u201ctranscriptome.\u201d<\/p>\n
Deficiencies in mRNA and non-coding RNA (ncRNA) are linked to certain neurodegenerative and neurodevelopmental disorders. If there is too little of a specific mRNA or ncRNA in the transcriptome, certain cellular functions may be degraded or disabled. Dr. Kieft is exploring a novel way to manage the transcriptome by slowing the decay of mRNA and ncRNA. Knowing that some enzymes that destroy the RNAs essentially \u201cchew\u201d it from one end to the other, Dr. Kieft used his understanding of how RNA molecules are structured and fold on themselves to create an engineered piece of exoribonuclease-resistant RNA (xrRNA) that, when introduced to compatible mRNA or ncRNA, combines and folds to form a \u201cblocking\u201d structure, literally changing the shape of the RNA by inserting a protrusion that stops the enzymes in their tracks.<\/p>\n
By slowing the decay of the target mRNA and ncRNA, Dr. Kieft sees the opportunity to manage their abundance within the transcriptome. Engineered xrRNAs could recognize just specific targets, link up with them, and create the protection, so researchers can increase the proportion of the target without changing how much is created. The approach has the advantage of being less disruptive to the host cell than unnaturally boosting mRNA, and the precision with which xrRNA can be engineered offers the potential to target multiple RNAs at once, and possibly even allow fine-tuning by precisely managing the rate of decay. Dr. Kieft sees this application, born of basic science studying RNA, as a potentially powerful research tool for neuroscientists, and perhaps even the foundation for therapies in the more distant future.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\tSuhasa Kodandaramaiah, Ph.D.,<\/a><\/strong> Benjamin Mayhugh Assistant Professor, Department of Mechanical Engineering, University of Minnesota Twin Cities <\/strong><\/p>\nRobot Assisted Brain-Wide Recordings in Freely Behaving Mice <\/em><\/strong><\/p>\nNeuroscientists studying brain activity during behaviors usually have to make a trade-off: They use miniaturized head-mounted neural sensors that are light enough to allow a subject animal to behave freely, but are lower resolution or can\u2019t monitor the whole brain. Or they use more powerful tools, which are far too heavy for subject animals and require other solutions, like immobilization while letting animals move on a treadmill, or even using virtual reality experiences that nonetheless limit the behavior of a subject.<\/p>\n
Dr. Kodandaramaiah is tackling the challenge with a robotic cranial exoskeleton that carries the weight of neural recording and monitoring hardware while still allowing the subject (in this case a mouse) to rotate its head in all three degrees: a full 360 degree turn in the yaw (horizontal rotation) axis, and about 50 degrees of motion in the pitch and roll axes, while moving around in an arena. The robot has three jointed arms arranged in a triangular configuration, suspended over the subject and meeting at the point of mounting on the head. Sensors in the mount will detect what motion the mouse is making and direct the robot to enable the motion with as little resistive force as possible, allowing the mouse to turn and move within an arena typically used for neuroscience experiments with all the necessary sensory equipment and wires from the implants supported by the robot.<\/p>\n
Taking out the need for miniaturization allows researchers to use whatever state-of-the art hardware is available, meaning a robot can theoretically be upgraded to use the latest technology soon after its introduction. To get to that point, Dr. Kodandaramaiah\u2019s team will go through several steps \u2013 engineering the exoskeleton; engineering the head-stage with its needed sensors plus high-density electrodes and cameras for external observation of eyes, whiskers and more; performing benchtop testing; tuning the robot to the inputs a mouse can deliver; determining how to introduce probes; and finally making live recording. With this mechanical underpinning, Dr. Kodandaramaiah hopes to help researchers get closer to the state where they can make detailed brain-wide neural recordings of freely behaving subjects over long timescales.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\t\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>\n\t\n\t\t\n\t\t\t2020-2021<\/h3>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>\n\t\n\t\t\n\t\t\t\u12a2\u126b \u12f3\u12e8\u122d \u1363 \u1352\u12a4\u127d.\u12f2.<\/a>\u1363 \u1228\u12f3\u1275 \u1355\u122e\u134c\u1230\u122d \u1363 \u12cb\u120b\u1235 \u12a4\u127d \u12ae\u1208\u122d \u12e8\u1263\u12ee\u121c\u12f2\u12ab\u120d \u12a2\u1295\u1302\u1290\u122a\u1295\u130d \u12ad\u134d\u120d \u1363 \u12e8\u1306\u122d\u1302\u12eb \u12e8\u1274\u12ad\u1296\u120e\u1302 \u1270\u124b\u121d \u12a5\u1293 \u12a2\u121a\u122a \u12e9\u1292\u1268\u122d\u1232\u1272<\/strong><\/p>\n"<\/em><\/strong>\u1260\u130a\u12dc\u12cd \u1363 \u1260\u1266\u1273 \u12a5\u1293 \u1260\u1263\u1205\u122a\u12eb\u1275 \u12cd\u1235\u1325 \u1275\u120d\u1245 \u1218\u1320\u1295 \u12eb\u1208\u12cd \u12e8\u1290\u122d\u126d \u12cd\u1202\u1266\u127d\u1295 \u1218\u1228\u1303\u12ce\u127d \u121b\u12c8\u12f3\u12f0\u122d \u201d<\/em><\/strong><\/p>\n\u12e8\u1290\u122d\u126d \u12cd\u1202\u1266\u127d\u1295 \u1260\u1275\u120b\u120d\u1245 \u12e8\u12a0\u12d5\u121d\u122e \u12ad\u134d\u120e\u127d \u120b\u12ed \u12e8\u1218\u1246\u1323\u1320\u122d \u12a5\u1293 \u12e8\u1218\u1218\u12dd\u1308\u1265 \u127d\u120e\u1273 \u12a5\u1305\u130d \u1260\u1323\u121d \u1265\u12d9 \u12e8\u1206\u1291 \u12e8\u12cd\u1202\u1265 \u12cd\u1324\u1276\u127d\u1295 \u12a0\u1235\u1308\u129d\u1277\u120d \u1363 \u12ed\u1205\u121d \u1260\u12d3\u1208\u121d \u120b\u12ed \u121d\u1295 \u12eb\u1205\u120d \u12e8\u1290\u122d\u126d \u1205\u12cb\u1236\u127d \u1218\u1228\u1303\u1295 \u1260\u12a0\u1295\u12f5 \u120b\u12ed \u1208\u121b\u12ab\u1270\u1275 \u12a0\u1265\u1228\u12cd\u1295 \u12a5\u1295\u12f0\u121a\u1230\u1229 \u12e8\u121a\u12eb\u1265\u122b\u122b \u1245\u1326\u127d\u1295 \u1208\u121b\u130d\u1298\u1275 \u12a0\u1235\u127d\u120f\u120d \u1361\u1361 \u1260\u12cd\u1202\u1265 \u1235\u1265\u1235\u1266\u127d \u12cd\u1235\u1325 \u12a0\u1290\u1235\u1270\u129b \u1218\u1320\u1295 \u12eb\u120b\u1278\u12cd \u1235\u122d\u12d3\u1270-\u1325\u1208\u1276\u127d\u1295 \u1260\u121b\u130d\u1298\u1275 \u12a0\u12f3\u12f2\u1235 \u1218\u123b\u123b\u120e\u127d \u1262\u1296\u1229\u1275\u121d \u1363 \u1228\u1305\u121d \u130a\u12dc \u122d\u12dd\u1218\u1275 \u12eb\u120b\u1278\u12cd \u12c8\u12ed\u121d \u1265\u12d9 \u12c8\u12ed\u121d \u1270\u1218\u1233\u1233\u12ed \u12c8\u12ed\u121d \u1270\u1218\u1233\u1233\u12ed \u1225\u122b\u12ce\u127d\u1295 \u12e8\u121a\u1348\u1273\u1270\u1291 \u12e8\u1270\u1208\u12eb\u12e9 \u130d\u1208\u1230\u1266\u127d\u1295 \u1363 \u12c8\u12ed\u121d \u1260\u1260\u123d\u1273 \u130d\u12db\u1276\u127d \u120b\u12ed \u121b\u1290\u1343\u1340\u122d \u12a0\u1201\u1295\u121d \u12a8\u1263\u12f5 \u1290\u12cd \u1361\u1361 \u12e8\u12a0\u1295\u130e\u120d \u12a5\u1295\u1245\u1235\u1243\u1234\u1295 \u1208\u1218\u1208\u12e8\u1275 \u12e8\u121b\u123d\u1295 \u1218\u121b\u122a\u12eb (ML) \u1260\u1218\u1320\u1240\u121d \u12e8\u1270\u121e\u12ad\u122e \u120d\u121d\u121d\u12f5 \u1260\u1265\u12d9 \u1275\u120b\u120d\u1245 \u12e8\u1290\u122d\u126d \u12f3\u1273\u1264\u1276\u127d \u12cd\u1235\u1325 \u1235\u122d\u12d3\u1270-\u1325\u1208\u1276\u127d\u1295 \u1208\u1218\u1208\u12e8\u1275 \u12c8\u12f0 \u12a0\u12f2\u1235 \u1218\u134d\u1275\u1204 \u12a5\u1295\u12f5\u1275\u12c8\u1235\u12f5 \u12a0\u12f5\u122d\u1313\u1273\u120d \u1361\u1361<\/p>\n
\u12e8\u12f6 \/ \u122d \u12f6yer \u1225\u122b \u12a5\u1295\u1235\u1233\u12cd \u1270\u129d\u1276 \u1363 \u1270\u129d\u1277\u120d \u1363 \u12a0\u1241\u121e \u1290\u1260\u122d \u12c8\u12ed\u121d \u1260\u1270\u1208\u12eb\u12e9 \u12a5\u1295\u1245\u1235\u1243\u1234\u12ce\u127d \u12c8\u12ed\u121d \u1263\u1205\u122a\u12ce\u127d \u12cd\u1235\u1325 \u12e8\u1270\u1218\u12d8\u1308\u1260 \u1218\u1206\u1291\u1295 \u1208\u1218\u1208\u12e8\u1275 \u12a8\u1270\u1230\u12e8\u1219 \u12e8\u1290\u122d\u126d \u12f3\u1273\u1264\u1276\u127d \u1218\u1228\u1303 \u1208\u121b\u12cd\u1323\u1275 \u12e8\u121b\u123d\u1295 \u1275\u121d\u1205\u122d\u1275 \u1235\u120d\u1270 \u1240\u1218\u122e\u127d\u1295 \u1218\u134d\u1320\u122d \u12eb\u12ab\u1275\u1273\u120d \u1361\u1361 \u12a0\u12f2\u1235 \u12e8\u1218\u1228\u1303 \u12a0\u1230\u1323\u1325 \u1270\u1290\u1233\u123d\u1290\u1275 \u12eb\u1208\u12cd \u12e8\u1202\u1233\u1265 \u1205\u130e\u127d \u12cd\u1202\u1261\u1295 \u12c8\u12f0 \u121b\u1218\u1323\u1320\u1295 \u12a5\u1295\u12f0 \u1218\u1290\u123b \u1290\u1325\u1265 \u12e8\u121a\u1218\u1208\u12a8\u1271\u1275 \u12e8\u1270\u1208\u12eb\u12e9 \u12e8\u12a0\u1295\u130e\u120d \u130d\u12db\u1276\u127d \u12e8\u1218\u1290\u1329 \u12e8\u1290\u122d\u126d \u12a5\u1295\u1245\u1235\u1243\u1234\u1295 \u1208\u121b\u12db\u1218\u12f5 \u1260\u1218\u1348\u1208\u130d \u1260\u1270\u1208\u12e8 \u12e8\u1218\u1228\u1303 \u1235\u1265\u1235\u1266\u127d \u12cd\u1235\u1325 \u1270\u1218\u1233\u1233\u12ed \u1235\u122d\u12d3\u1270-\u1325\u1208\u1276\u127d\u1295 \u1208\u1218\u1208\u12e8\u1275 \u1235\u120d\u1270 \u1240\u1218\u1229\u1295 \u12ed\u1218\u122b\u1209 \u1361\u1361 \u12e8\u1290\u122d\u126d \u12a5\u1295\u1245\u1235\u1243\u1234\u1295 \u121b\u1218\u127b\u1278\u1275 \u12e8\u1290\u122d\u126d \u1225\u122d\u12d3\u1276\u127d \u12a8\u122d\u12d5\u1230-\u1263\u1205\u122a\u12cd \u1201\u1294\u1273 \u12a5\u1293 \u1201\u1294\u1273 \u130b\u122d \u12a5\u1295\u12f4\u1275 \u12a5\u1295\u12f0\u121a\u12db\u1218\u12f1 \u12a5\u1295\u12f2\u1201\u121d \u1219\u1235\u1293\u1295 \u1260\u12f5\u121d\u1345 \u1218\u12a8\u120b\u12a8\u120d\u1295 \u12eb\u1233\u12eb\u120d \u1363 \u12a5\u1293\u121d \u1208\u1260\u1208\u1320 \u1283\u12ed\u1208\u129b \u1275\u1295\u1270\u1293 \u1274\u12ad\u1292\u12ae\u127d \u12c8\u1233\u129d \u12a5\u122d\u121d\u1303 \u12ed\u1230\u1323\u120d \u1361\u1361<\/p>\n
\u12e8\u12f6 \/ \u122d \u12f6\u1208\u122d \u1201\u1208\u1270\u129b \u12d3\u120b\u121b \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u1290\u122d\u126d \u1290\u122ds\u127d \u120b\u12ed \u1260\u1290\u122d\u126d \u12a5\u1295\u1245\u1235\u1243\u1234 \u12a0\u1320\u1243\u120b\u12ed \u1208\u12cd\u1326\u127d \u12a5\u1295\u12f4\u1275 \u12a0\u1235\u1270\u12cb\u1345\u12a6 \u12a5\u1295\u12f3\u12f0\u1228\u1309 \u12a5\u1293 \u12e8\u1270\u12c8\u1230\u1291 \u12e8\u12a0\u1295\u130e\u120d \u1201\u1294\u1273\u12ce\u127d\u1295 \u1208\u1218\u1270\u1295\u1260\u12ed \u1325\u1245\u121d \u120b\u12ed \u1218\u12cb\u120d \u12a5\u1295\u12f0\u121a\u127d\u1209 \u12a5\u1295\u12f2\u1308\u1290\u12d8\u1261 \u12ed\u1228\u12f3\u120d \u1361\u1361 \u12e8\u1263\u1205\u122a\u12ed \u120d\u12e9\u1290\u1276\u127d \u12c8\u12f0 \u1270\u1208\u12e9 \u12e8\u1215\u12cb\u1235 \u12d3\u12ed\u1290\u1276\u127d \u1218\u1218\u1208\u123b \u120b\u12ed \u1218\u12cb\u120d \u12a0\u1208\u1218\u127b\u1209\u1295 \u1363 \u12a5\u1293 \u1260\u1218\u1228\u1303 \u124b\u1276\u127d \u12cd\u1235\u1325 \u12e8\u1273\u12e9\u1275 \u120d\u12e9\u1290\u1276\u127d \u1260\u12a5\u12eb\u1295\u12f3\u1295\u12f1 \u12a5\u1295\u1235\u1233 \u120b\u12ed \u120d\u12e9\u1290\u1275\u1295 \u1208\u1218\u1208\u12e8\u1275 \u1325\u1245\u121d \u120b\u12ed \u12a5\u1295\u12f2\u12cd\u1209 \u1325\u1293\u1271 \u12e8\u1260\u1208\u1320 \u121d\u122d\u121d\u122d \u12eb\u12f0\u122d\u130b\u120d \u1361\u1361 \u1275\u120b\u120d\u1245 \u12e8\u1290\u122d\u126d \u12e8\u1290\u122d\u126d \u12f3\u1273\u1264\u1276\u127d\u1295 \u12e8\u1218\u1208\u12e8\u1275 \u12a5\u1293 \u12e8\u121b\u1290\u1343\u1340\u122d \u127d\u120e\u1273 \u12e8\u12a0\u1295\u130e\u120d \u12e8\u1290\u122d\u126d \u1225\u122d\u12d3\u1275\u1295 \u1260\u123d\u1273 \u1260\u12a0\u1295\u130e\u120d \u1218\u1228\u1303 \u1202\u12f0\u1275 \u120b\u12ed \u121d\u1295 \u12eb\u1205\u120d \u12a5\u1295\u12f0\u121a\u130e\u12f3 \u1260\u1218\u1320\u1246\u121d \u1260\u1292\u12cd\u122e\u120e\u1302\u12ab\u120d \u121d\u122d\u121d\u122d \u12cd\u1235\u1325 \u1275\u120d\u1245 \u12cb\u130b \u12ed\u1296\u1228\u12cb\u120d \u1361\u1361<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\t\u122a\u12aa\u12aa \u1219\u1208\u122d \u1363 \u1352\u12a4\u127d.\u12f2.<\/a>\u1363 \u1260\u12a4\u120c\u12ad\u1275\u122a\u12ad \u121d\u1205\u1295\u12f5\u1235\u1293 \u12a5\u1293 \u1260\u12ae\u121d\u1352\u12cd\u1270\u122d \u1233\u12ed\u1295\u1235 \u1228\u12f3\u1275 \u1355\u122e\u134c\u1230\u122d \u1363 \u1260\u12ab\u120a\u134e\u122d\u1292\u12eb \u12e9\u1292\u1268\u122d\u1232\u1272 - \u1260\u122d\u12ad\u120c\u12ed<\/strong><\/p>\n"<\/strong><\/em>\u1260\u123a\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u12e8\u1290\u122d\u126d \u1234\u120e\u127d\u1295 \u1208\u12a6\u1355\u120e\u1300\u1295\u1272\u12ab\u12ca \u1241\u1325\u1325\u122d \u12e8\u121a\u1206\u1295 \u12a8\u134d\u1270\u129b \u134d\u1325\u1290\u1275 \u12eb\u1208\u12cd \u12e8\u1206\u120e\u130d\u122b\u134a \u1218\u1223\u122a\u12eb \u201d<\/em><\/strong><\/p>\n\u12a6\u1355\u1276\u1304\u1294\u1272\u12ad\u1235 - \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u1348\u1208\u1309\u1275 \u130a\u12dc \u12a5\u1295\u12f2\u1220\u1229\u1263\u1278\u12cd \u12c8\u12ed\u121d \u12dd\u121d \u12a5\u1295\u12f2\u120d\u120b\u1278\u12cd \u12e8\u1290\u122d\u126d \u1234\u120e\u127d\u1295 \u1260\u12ad\u1265 \u1265\u122d\u1203\u1295-\u1270\u12ae\u122d \u12a5\u1295\u12f2\u1206\u1291 \u1260\u121b\u12f5\u1228\u130d - \u12a6\u12cd\u1276\u120e\u1302\u12ab\u12ca \u12e8\u1290\u122d\u126d-\u121d\u122d\u121d\u122d \u121d\u122d\u121d\u122d\u1295 \u1240\u12ed\u1228\u12cb\u120d \u1361\u1361 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1265\u122d\u1203\u1295\u1295 \u12c8\u12f0 3 \u12f2 \u1202\u120e\u130d\u122b\u121d \u12a8\u121a\u1240\u12eb\u12ed\u1260\u1275 \u12e8\u1266\u1273 \u1265\u122d\u1203\u1295 \u121e\u12f2\u12eb\u1270\u122e\u127d \u130b\u122d \u12e8\u1270\u1323\u1218\u1229 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u1270\u1293\u1325\u120d \u1260\u12a0\u1295\u12f1 \u1263\u1208 \u1236\u1235\u1275 \u12a0\u1245\u1323\u132b\u12ca \u12ad\u120d\u120d \u12cd\u1235\u1325 \u12e8\u121a\u1308\u1299\u1275\u1295 \u1260\u122d\u12ab\u1273 \u12e8\u1290\u122d\u126d \u1234\u120e\u127d\u1295 \u1260\u130d\u120b\u1278\u12cd \u1218\u1246\u1323\u1320\u122d \u12ed\u127d\u120b\u1209 \u1361\u1361 in vivo<\/em>. \u130d\u1295 \u12a5\u1235\u12a8\u12da\u1205 \u12f5\u1228\u1235 \u1260\u1270\u1348\u1325\u122e \u1260\u1270\u1348\u1325\u122e \u1260\u12a0\u1295\u130e\u120d \u12cd\u1235\u1325 \u1260\u121a\u1308\u1299 \u12e8\u1290\u122d\u126d \u134d\u1325\u1290\u1276\u127d \u120b\u12ed \u12e8\u1290\u122d\u126d \u1234\u120e\u127d\u1295 \u1208\u1218\u1246\u1323\u1320\u122d \u12e8\u121a\u12eb\u1235\u127d\u120d \u12e8\u1206\u120e\u130d\u122b\u134a \u1355\u122e\u1304\u12ad\u1275 \u12e8\u1208\u121d \u1361\u1361<\/p>\n\u12f6\u12ad\u1270\u122d \u1219\u1208\u122d \u12ed\u1205\u1295\u1295 \u127d\u130d\u122d \u1208\u1218\u1245\u1228\u134d \u12e8\u1206\u120e\u130d\u122b\u134a \u1355\u122e\u1304\u12ad\u1275 (\u12f2\u12db\u12ed\u1290\u122d) \u12f2\u12db\u12ed\u1295 \u1260\u121b\u12d8\u130b\u1300\u1275 \u120b\u12ed \u1290\u12cd \u1361\u1361 \u1218\u1223\u122a\u12eb\u12cb \u12e8\u1206\u120e\u130d\u122b\u134a\u12ad \u1265\u122d\u1203\u1295 \u121d\u1235\u120e\u127d\u1295 \u1260\u1234\u12ae\u1295\u12f5 \u1260 10,000 \u12ad\u1348\u134e\u127d (\u12a4\u127d.\u12dc.) \u12cb\u130b\u12ce\u127d\u1295 \u1260\u12e5\u1228\u1275 \u12ed\u120d\u1243\u120d \u1361\u1361 \u1265\u12d9 \u12e8\u12c8\u1245\u1271-\u1275\u12cd\u120d\u12f5 \u1274\u120c\u126a\u12e5\u1296\u127d \u1208\u12a0\u1295\u12f5 \u1230\u12a8\u1295\u12f5 60 \u134d\u122c\u121e\u127d\u1295 \u12eb\u12f5\u1233\u1209 \u1363 \u1208\u121b\u1290\u1343\u1340\u122d \u12a5\u1293 \u1260 500 Hz \u12a8\u134d\u1270\u129b \u1325\u122b\u1275 \u120b\u120b\u1278\u12cd \u12e8\u1206\u120e\u130d\u122b\u134a\u12ad \u1218\u1233\u122a\u12eb\u12ce\u127d \u1260\u120b\u1240 \u12f0\u1228\u1303 \u120b\u12ed \u12ed\u1308\u129b\u1209 \u1361\u1361 \u12ed\u1205 \u12a8\u134d\u1270\u129b \u12e8\u121b\u12f0\u1235 \u12f0\u1228\u1303 \u1260\u1270\u1348\u1325\u122e\u12cd \u12e8\u1290\u122d\u126d \u121d\u120d\u12ad\u1275 \u121d\u120d\u12ad\u1275\u1295 \u1208\u1218\u12ae\u1228\u1305 \u12a0\u1235\u1348\u120b\u130a \u1290\u12cd \u1363 \u12ed\u1205\u121d \u1260\u1230\u12a8\u1295\u12f5 1\/1000 \u129b \u130a\u12dc \u120a\u1348\u1320\u122d \u12e8\u121a\u127d\u120d \u12a5\u122d\u121d\u1303\u12ce\u127d\u1295 (\u12a8\u1245\u12f3\u1234 \u1218\u1320\u1295 \u130b\u122d \u1232\u1290\u1343\u1340\u122d \u12a8 1000 Hz \u130b\u122d \u12a5\u12a9\u120d \u1290\u12cd) \u1361\u1361 \u12a5\u1293 \u1260\u1274\u120c\u126a\u12e5\u1296\u127d \u12cd\u1235\u1325 \u12a8\u134d \u12eb\u1208 \u12cb\u130b\u12ce\u127d \u12a0\u1320\u122d \u12eb\u1208 \u121d\u1235\u120e\u127d\u1295 \u12a5\u1295\u12f0\u121a\u1328\u121d\u1229 \u1201\u1209 \u1363 10,000 \u12a4\u127d \u1204\u12dd hologram \u12e8\u120b\u1240 \u1275\u12ad\u12ad\u1208\u129b\u1290\u1275 \u12ed\u1230\u1323\u120d\u1362<\/p>\n
\u1260\u1292\u12cd\u122e\u1274\u12ad\u1296\u120e\u1302\u12cd \u120b\u12ed \u12eb\u1270\u12ae\u1228 \u12a4\u120c\u12ad\u1275\u122a\u12ad \u1218\u1210\u1295\u12f2\u1235 \u1363 \u12f2\u12db\u12ed\u1295 \u1260\u121a\u12eb\u12f0\u122d\u1309\u1260\u1275 \u1363 \u1260\u121a\u121e\u12ad\u1229\u1260\u1275 \u12a5\u1293 \u1218\u1233\u122a\u12eb\u12ce\u1279\u1295 \u134d\u120b\u130e\u1276\u127b\u1278\u12cd\u1295 \u12a5\u1295\u12f2\u12eb\u121f\u120b \u1260\u121a\u12eb\u12f0\u122d\u130d\u1260\u1275 \u130a\u12dc \u12e8\u1290\u122d\u126d \u1210\u12aa\u121e\u127d\u1295 \u1260\u1218\u12f0\u1260\u129b\u1290\u1275 \u12eb\u121b\u12ad\u122b\u120d \u1361\u1361 \u1218\u1223\u122a\u12eb\u12cd \u12e8 3 \u12f2 \u1265\u122d\u1203\u1295 \u1201\u1294\u1273\u12ce\u127d\u1295 \u1208\u1270\u12c8\u1230\u1291 \u12a0\u12ab\u1263\u1262\u12ce\u127d \u12a5\u1293 \u1260\u1325\u1243\u1245\u1295 \u1218\u1235\u1273\u12c8\u1276\u127d \u1260\u12a4\u120c\u12ad\u1275\u122a\u12ad \u12a5\u1295\u1245\u1235\u1243\u1234 \u12a0\u121b\u12ab\u129d\u1290\u1275 \u12c8\u12f0 \u121a\u12eb\u1233\u12e8\u12cd \u121b\u12ed\u12ad\u122e\u121a\u122a\u12e8\u122d \u12f5\u122d\u12f5\u122d \u12ed\u1320\u1240\u121b\u120d \u1363 \u12a8\u12da\u12eb \u1265\u122d\u1203\u1291 \u1260\u1270\u12a8\u1273\u1273\u12ed \u120c\u1295\u1236\u127d \u12a0\u121b\u12ab\u12ed\u1290\u1275 \u12ed\u1308\u1208\u133b\u120d \u1361\u1361 \u1218\u122d\u1203\u130d\u1265\u1229 \u1201\u1208\u1275 \u12f5\u122d\u12f5\u122e\u127d\u1295 \u12eb\u1240\u1348 \u12a5\u1293 \u12eb\u12d8\u130b\u1303\u120d - \u1208\u1219\u12a8\u122b \u12a5\u1293 \u1208\u1345\u1295\u1230-\u1200\u1233\u1265 \u12a0\u1290\u1235 \u12eb\u1208 \u12a0\u12f0\u122b\u12f0\u122d \u1363 \u12a5\u1293 \u1208\u1218\u1208\u12ab\u1275 \u12a5\u1293 \u1208\u1218\u1208\u12ab\u1275 \u12a8\u121a\u12eb\u1235\u1348\u120d\u1309\u1275 \u1270\u1313\u12f3\u129d \u1290\u1302\u12ce\u127d \u12a5\u1293 \u1218\u1246\u1323\u1320\u122a\u12eb\u12ce\u127d \u130b\u122d \u12a0\u1295\u12f5 \u1275\u1295\u123d \u12a0\u12f0\u122b\u12f0\u122d\u1362 \u1260\u1218\u1328\u1228\u123b\u121d \u12e8\u12f6 \/ \u122d \u1219\u1209\u1208 \u1261\u12f5\u1295 \u1261\u12f5\u1295 \u1219\u1209 \u1260\u1219\u1209 \u12e8\u1270\u1235\u1270\u12ab\u12a8\u1208 \u12e8\u1266\u1273 \u1265\u122d\u1203\u1295 \u121e\u12f1\u1270\u122d \u12eb\u1235\u1308\u129b\u120d \u1361\u1361 \u12ed\u1205 \u1218\u1223\u122a\u12eb \u1270\u1218\u122b\u121b\u122a\u12ce\u127d\u1295 \u12e8\u1290\u122d\u126d \u130d\u1295\u1299\u1290\u1276\u127d\u1295 \u12e8\u1218\u1246\u1323\u1320\u122d \u12a5\u1293 \u12e8\u1218\u1348\u1270\u123d \u12eb\u120d\u1270\u1208\u1218\u12f0 \u127d\u120e\u1273 \u12ed\u1230\u1323\u1278\u12cb\u120d \u1270\u1265\u120e \u1270\u1235\u134b \u1270\u12f0\u122d\u1313\u120d \u1361\u1361<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\t\u12ab\u12ed \u12da\u1295, \u1352\u12a4\u127d.<\/a>\u1363 \u1203\u12cb\u122d\u12f5 \u12a5\u1293 \u130d\u12cc\u1295 \u120e\u122a \u12e8\u1263\u12ee\u120e\u1302 \u1355\u122e\u134c\u1230\u122d \u1363 \u12ab\u120a\u134e\u122d\u1292\u12eb \u12e8\u1274\u12ad\u1296\u120e\u1302 \u1270\u124b\u121d \u1232\u1218\u1273<\/strong><\/p>\n"<\/em><\/strong>\u121e\u12f1\u120d \u12a2\u1295\u12db\u12ed\u121d\u1272\u12ad \u1263\u122d\u12ae\u12f2\u1295\u130d \u201d<\/em><\/strong><\/p>\n\u1265\u12d9 \u12e8\u1290\u122d\u126d \u1260\u123d\u1273 \u121d\u122d\u1218\u122b\u12ce\u127d \u12e8\u1215\u12cb\u1235 \u1308\u133d\u1273\u1295 \u12e8\u121a\u12a8\u120b\u12a8\u1209 \u1340\u1228-\u1230\u12cd \u12a5\u1293 \u12e8\u1270\u1240\u1263\u12ee\u127d \u1275\u1295\u1273\u1294 \u12eb\u12ab\u1275\u1273\u1209\u1362 \u12f0\u130d\u121e\u121d \u1235\u1208 \u12e8\u1290\u122d\u126d \u120d\u121b\u1275 \u12a5\u1293 \u1270\u130d\u1263\u122d \u130d\u1295\u12db\u1264 \u1235\u1208 \u12a5\u12cd\u1240\u1275 \u12ed\u1320\u12ed\u1243\u120d in vivo<\/em> \u12e8\u1215\u12cb\u1235 \u12c8\u1208\u120d \u1355\u122e\u1272\u1296\u127d \u1218\u12ab\u12a8\u120d \u130d\u1295\u1299\u1290\u1276\u127d\u1362 \u12a5\u12eb\u1295\u12f3\u1295\u12f1 \u1355\u122e\u1272\u1295 \u12e8\u1270\u1208\u12eb\u12e9 \u1263\u12ee\u12ac\u121a\u12ab\u12ca \u1263\u1205\u122a\u12ce\u127d \u1235\u120b\u1209\u1275 \u1355\u122e\u1272\u1296\u127d\u1295 \u12e8\u121a\u12eb\u12ab\u1275\u1271 \u12a8\u134d\u1270\u129b \u12e8\u121d\u122d\u121d\u122d \u1219\u12a8\u122b\u12ce\u127d \u130a\u12dc\u1295 \u12e8\u121a\u12c8\u1235\u12f5 \u12a5\u1293 \u12cd\u1235\u1265\u1235\u1265 \u1293\u1278\u12cd\u1362 \u12e8\u1290\u122d\u126d \u1233\u12ed\u1295\u1235 \u121d\u122d\u121d\u122d \u12a0\u12f3\u12f2\u1235 \u12d5\u12f5\u120e\u127d\u1295 \u1208\u1218\u12ad\u1348\u1275 \u1208\u121b\u1308\u12dd \u12f6\u12ad\u1270\u122d \u12d8\u12ed\u1295 \u12a5\u1293 \u12e8\u12a5\u1231 \u1261\u12f5\u1295 \u12e8\u1270\u1208\u12eb\u12e9 \u1355\u122e\u1272\u1296\u127d\u1295 \u201c\u1263\u122d\u12ae\u12f5\u201d \u12e8\u1270\u1208\u12eb\u12e9 \u1355\u122e\u1272\u1296\u127d\u1295 (modcode) \u1218\u1295\u1308\u12f5 \u1260\u1218\u134d\u1320\u122d \u1270\u1218\u122b\u121b\u122a\u12ce\u127d\u1295 \u1270\u1208\u12cb\u12cb\u132d \u12e8\u1218\u1233\u122a\u12eb \u12aa\u1235 \u12ed\u1230\u1323\u1209 \u1361\u1361<\/p>\n\u1263\u122d\u12ae\u12f2\u1295\u130d \u1260\u1240\u120b\u120d \u1245\u122d\u1345 \u12e8\u1304\u1294\u1272\u12ad \u12a0\u1218\u120d\u12ab\u127e\u127d\u1295 \u12c8\u12f0 \u121e\u1208\u12aa\u12cd\u120e\u127d \u12cd\u1235\u1325 \u121b\u1235\u1308\u1263\u1275 \u12a5\u1293 \u12a8\u12db\u121d \u1219\u12a8\u122b\u12cd \u1260\u128b\u120b \u12e8\u1275\u129e\u1279 \u121e\u1208\u12aa\u12cd\u120e\u127d \u1260\u12a0\u1295\u12f5 \u120b\u12ed \u12a5\u1295\u12f0\u1270\u1320\u1246\u1219 \u1208\u121b\u12c8\u1245 \u12a5\u1290\u12da\u1205\u1295 \u12a0\u1218\u120d\u12ab\u127e\u127d \u1218\u1348\u1208\u130d\u1295 \u12eb\u12ab\u1275\u1273\u120d\u1362 \u12a8\u1273\u120b\u1245 \u1235\u12ac\u1275 \u130b\u122d \u12a8\u1292\u12cd\u12ad\u120a\u12ad \u12a0\u1232\u12f5 \u130b\u122d \u1325\u1245\u121d \u120b\u12ed \u12cd\u120f\u120d\u1362 \u1355\u122e\u1272\u1296\u127d \u12ed\u1260\u120d\u1325 \u12e8\u1270\u12c8\u1233\u1230\u1261 \u1293\u1278\u12cd \u1363 \u130d\u1295 \u1265\u12d9 \u130a\u12dc \u12e8\u1355\u122e\u1272\u1295 \u1270\u130d\u1263\u122d\u1295 \u12e8\u121a\u1240\u12ed\u122d \u12ac\u121a\u12ab\u12ca \u12a0\u124b\u122b\u132d \u1218\u1295\u1308\u12f5 \u1233\u12ed\u1296\u122b\u1278\u12cd \u1208\u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u123a\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u12e8\u134d\u120b\u130e\u1275 \u1355\u122e\u1272\u1296\u127d\u1295 \u1208\u1218\u1208\u12eb\u12e8\u1275 \u12e8\u121a\u12eb\u1235\u127d\u120d \u1218\u1295\u1308\u12f5 \u12a0\u120d\u1290\u1260\u1228\u121d\u1362 \u12f6 \/ \u122d \u12da\u1295 \u12a8 \u201cHUH-domain\u201d \u12a2\u1295\u12db\u12ed\u121e\u127d \u130b\u122d \u12e8\u1270\u1246\u122b\u1299 \u12a8\u134d\u1270\u129b-\u12e8\u130d\u1295\u1299\u1290\u1275 \u1355\u122e\u1272\u1295 \u121b\u12eb\u12eb\u12e3 \u121e\u1301\u120e\u127d\u1295 \u12e8\u12eb\u12d9 \u12e8\u1305\u121d\u120b \u1355\u122e\u1272\u1296\u127d\u1295 \u1260\u1218\u1320\u1240\u121d \u12ed\u1205\u1295\u1295 \u1270\u130d\u12f3\u122e\u1275 \u12a5\u12eb\u1238\u1290\u1349 \u1290\u12cd \u1361\u1361 \u1270\u1323\u1263\u1242 \u121e\u1301\u120e\u1279 \u1263\u122d\u12ae\u12ae\u12ae\u127d\u1295 \u12a8\u1340\u1228-\u1270\u1215\u12cb\u1235\u12eb\u1295 \u1363 \u12a8\u1262\u12ee\u1273\u12ed\u121a\u12ad \u1355\u122e\u1272\u1296\u127d \u12a5\u1293 \u1355\u122e\u1272\u1296\u127d \u130b\u122d \u12e8\u1270\u1233\u1230\u1229 \u12e8\u1218\u12eb\u12e3 \u1218\u1208\u12eb\u12ce\u127d \u130b\u122d \u12a5\u1295\u12f2\u1323\u1260\u1241 \u12eb\u1235\u127d\u120b\u1278\u12cb\u120d \u1361\u1361 \u12ed\u1205 \u1208\u12a0\u1265\u12db\u129b\u12ce\u1279 \u12e8\u1290\u122d\u126d \u1210\u12aa\u121e\u127d \u12a8\u134d\u1270\u129b \u134d\u120b\u130e\u1275 \u12eb\u120b\u1278\u12cd \u1355\u122e\u1272\u1296\u127d \u1218\u12f3\u1228\u123b\u1295 \u12ed\u1230\u1323\u120d \u1361\u1361 \u1260\u1270\u1328\u121b\u122a\u121d \u1218\u122d\u1203\u130d\u1265\u1229 \u1293\u1296\u12a0\u134d\u120a\u12ab\u120d \u1235\u12ab\u1290\u122e\u127d\u1295 \u12e8\u121a\u12ed\u12d9 60 \u12e8\u121b\u12eb\u12eb\u12e3 \u1290\u1325\u1266\u127d\u1295 \u1260\u1270\u1218\u1233\u1233\u12ed \u130a\u12dc \u12a8\u1263\u122d\u12ae\u12f5 \u12a5\u1293 \u12a8\u134d\u120b\u130e\u1275 \u1355\u122e\u1272\u1296\u127d \u130b\u122d \u121b\u12eb\u12eb\u12dd \u12ed\u127d\u120b\u120d \u1361\u1361 \u12a5\u1290\u12da\u1205 \u1245\u122d\u134a\u1276\u127d \u12e8\u130d\u1295\u1299\u1290\u1276\u127d\u1295 \u121d\u120d\u12a8\u1273 \u12eb\u123b\u123d\u120b\u1209 - \u1260\u12a5\u12eb\u1295\u12f3\u1295\u12f1 \u1218\u12cb\u1245\u122d \u120b\u12ed \u12eb\u1209 \u1260\u122d\u12ab\u1273 \u1355\u122e\u1272\u1296\u127d \u1232\u1308\u1293\u1299 \u12f0\u12ab\u121b \u130d\u1295\u1299\u1290\u1276\u127d \u1320\u1295\u12ab\u122b \u12ed\u1206\u1293\u1209\u1362<\/p>\n
\u12e8\u12f6\u12ad\u1270\u122d \u12dc\u1295 \u1355\u122e\u1300\u12ad\u1275 \u1355\u122e\u1272\u1296\u127d\u1295 \u12a5\u1293 \u1218\u1228\u1303\u12ce\u127d\u1295 \u12e8\u121a\u12eb\u1240\u122d\u1261 \u1260\u122d\u12ab\u1273 \u12a8\u134d\u1270\u129b-\u1290\u1320\u120b-\u1290\u1320\u120b-\u1290\u1320\u120b-\u1234\u120d \u1245\u12f0\u121d-\u1270\u12a8\u1270\u120d \u1219\u12a8\u122b\u12ce\u127d\u1295 \u1260\u121b\u12ab\u1204\u12f5 \u120b\u12ed \u12e8\u1270\u1233\u1270\u1349 \u1355\u122e\u1276\u12ae\u120e\u127d\u1295 \u12a5\u1293 \u1202\u12f0\u1276\u127d\u1295 \u121b\u12d8\u130b\u1300\u1275 \u12ed\u1300\u121d\u122b\u120d \u1361\u1361 \u12a5\u1290\u12da\u1205 \u1260\u12a0\u1295\u12f5 \u1234\u120d \u120b\u12ed \u12e8\u1270\u12c8\u1230\u1291 \u12e8\u1270\u12c8\u1230\u1291 \u1270\u1240\u1263\u12ee\u127d \u1270\u1240\u1263\u12ee\u127d \u12a0\u1308\u120b\u1208\u1345\u1295 \u1208\u1218\u1218\u120d\u12a8\u1275 \u1363 \u1208\u12a0\u1295\u12f3\u1295\u12f5 \u1355\u122e\u1272\u1296\u127d \u1232\u130b\u1208\u1321 \u1260\u1234\u120e\u127d \u120b\u12ed \u12e8\u121a\u12f0\u1228\u1309 \u1208\u12cd\u1326\u127d\u1295 \u1208\u1218\u1218\u120d\u12a8\u1275 \u1363 \u1260\u12a0\u1295\u130e\u120d \u1215\u1265\u1228 \u1215\u12cb\u1233\u1275 \u12cd\u1235\u1325 \u1265\u12d9 \u12a0\u1295\u1272\u1302\u1296\u127d\u1295 \u1208\u1218\u1308\u1218\u1275 \u1363 \u12e8\u1355\u122e\u1272\u1296\u127d \u1265\u12db\u1275 \u1218\u1235\u1270\u130b\u1265\u122d\u1295 \u1208\u121b\u12e8\u1275 \u12a5\u1293 \u1208 \u201c\u12c8\u120b\u1305 \u12a0\u120d\u1263\u201d \u1355\u122e\u1272\u1296\u127d \u1270\u1240\u1263\u12ee\u127d\u1295 \u1218\u1208\u12e8\u1275 \u1361\u1361 \u1263\u1208\u121e\u12eb\u1290\u1271 \u1363 \u1240\u120b\u120d\u1290\u1271 \u12a5\u1293 \u1260\u12a0\u1295\u12f5 \u130a\u12dc \u1260\u122d\u12ab\u1273 \u1355\u122e\u1272\u1296\u127d \u12a5\u1295\u12f2\u1230\u1229 \u12e8\u1218\u134d\u1240\u12f5 \u127d\u120e\u1273\u12cd \u121d\u1235\u130b\u1293 \u12ed\u130d\u1263\u12cd\u1293 \u12f6 \/ \u122d \u12da\u1295 \u12e8\u12a5\u1231 \u12e8\u1218\u122d\u1308\u132b \u1235\u122d\u12d3\u1271 \u12a5\u1290\u12da\u1205\u1295 \u12a5\u1293 \u120c\u120e\u127d \u1260\u122d\u12ab\u1273 \u12e8\u1290\u122d\u126d \u1205\u120a\u1293 \u1219\u12a8\u122b\u12ce\u127d\u1295 \u12eb\u134b\u1325\u1293\u120d \u12a5\u1293 \u12eb\u134b\u1325\u1293\u120d \u1361\u1361<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\t2019-2020<\/h3>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>\n\t\n\t\t\n\t\t\t\u1308\u122d\u120d \u12a2\u126d\u122e\u1295 \u1363 \u12a4\u121d.\u12a0\u122d. \u1363 \u1352. \u12f2. \u1363<\/a> \u1228\u12f3\u1275 \u1355\u122e\u134c\u1230\u122d \u1363 \u12e8\u1230\u12cd \u12d8\u1228-\u1218\u120d (\u1305\u1295) \u12a5\u1293 \u1302\u12a6\u120e\u1302\u12ad\u1235 \u121b\u12d5\u12a8\u120d \u12e8\u1292\u12cd \u12ee\u122d\u12ad \u12e9\u1292\u1268\u122d\u1232\u1272 \u120b\u1295\u130d\u1276\u1295 \u1324\u1293<\/strong><\/p>\n\u201c\u1275\u12d5\u12ed\u1295\u1275-\u12a8\u134d\u1270\u129b \u12e8\u12a0\u12a5\u121d\u122e \u12d8\u1228-\u1218\u120d\u1218\u12f5\u1295 \u1208\u1230\u1265\u12a0\u12ca \u134d\u1230\u1275 \u12a0\u1230\u1323\u1325 \u1290\u1320\u120b-\u1234\u120d \u1263\u1208\u1265\u12d9-\u1274\u12ad\u1296\u120e\u1302 \u1274\u12ad\u1296\u120e\u1302\u201d<\/em><\/strong><\/p>\n\u12a0\u1295\u12f5 \u12a5\u12eb\u1295\u12f3\u1295\u12f1 \u134d\u1321\u122d \u12a0\u1295\u12f5 \u1290\u1320\u120b \u12f2\u1293 \u201c\u1218\u1218\u122a\u12eb\u12ce\u127d\u201d \u12eb\u1208\u12cd \u12a0\u1295\u12f5 \u1205\u12cb\u1235 \u12e8\u121a\u1300\u121d\u122d\u1260\u1275 \u12a0\u1295\u12f5 \u1215\u12cb\u1235 \u12a5\u1295\u12f0\u1206\u1290 \u12e8\u121a\u1273\u12c8\u1245 \u1290\u1308\u122d \u130d\u1295 \u12a0\u1295\u12f5 \u1205\u12cb\u1235 \u1260\u123a\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u1260\u1262\u120a\u12ee\u1295 \u12e8\u121a\u1246\u1320\u1229 \u1205\u12cb\u1233\u1275\u1295 \u1328\u121d\u122e - \u12a0\u1201\u1295\u121d \u1260\u12a0\u12a5\u121d\u122e \u12cd\u1235\u1325 \u1308\u1293 \u12a0\u120d\u1273\u12c8\u1240\u121d \u1361\u1361 \u12e8\u12f6\u12ad\u1270\u122d \u12a2\u126a\u1295\u1292 \u121d\u122d\u121d\u122d \u12e8\u1273\u1218\u1240 \u1215\u12cb\u1233\u1275 \u1260\u1230\u12cd \u12a0\u1295\u130e\u120d \u12cd\u1235\u1325 \u120b\u1209\u1275 \u1260\u1218\u1276\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u12e8\u130e\u120d\u121b\u1233 \u1234\u120e\u127d \u12d3\u12ed\u1290\u1276\u127d \u12e8\u1275\u129b\u12cd\u1295 \u12e8\u12d8\u122d \u1205\u12cb\u1235 \u1355\u122e\u1272\u1296\u127d \u12a5\u1295\u12f0\u121a\u1230\u1321 \u1260\u1218\u130d\u1208\u133d \u12ed\u1205\u1295\u1295 \u1202\u12f0\u1275 \u12e8\u121a\u12eb\u1265\u122b\u122b \u201c\u1275\u122a\u1355\u1232\u201d \u12e8\u1270\u1263\u1208 \u1274\u12ad\u1296\u120e\u1302\u1295 \u121b\u12f3\u1260\u122d \u1290\u12cd \u1361\u1361<\/p>\n
\u1274\u12ad\u1296\u120e\u1302\u12cd \u12e8\u1230\u12ce\u127d\u1295 \u12e8\u12a0\u1295\u130e\u120d \u12a5\u12f5\u1308\u1275 \u12e8\u121a\u12eb\u1320\u1291 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u12e8\u121a\u12eb\u130b\u1325\u121f\u1278\u12cd\u1295 \u1241\u120d\u134d \u1241\u120d\u134d \u1309\u12f3\u12ee\u127d \u120a\u1348\u1273 \u12ed\u127d\u120b\u120d \u1361\u1361 \u1218\u1235\u1218\u122e\u127d\u1295 \u1260\u1218\u1348\u1208\u130d (\u12d8\u12f4\u12ce\u127d\u1295 \u12c8\u12f0 \u12eb\u120d\u1260\u1241 \u12a5\u1295\u1235\u1233\u1275 \u1215\u12cb\u1233\u1275 \u12cd\u1235\u1325 \u1260\u121b\u1235\u1308\u1263\u1275 \u12a5\u1293 \u12a8\u12da\u12eb \u12a0\u1218\u120d\u12ab\u127e\u127d \u12c8\u12f0 \u12d8\u122e\u127b\u1278\u12cd \u12a5\u1295\u12f4\u1275 \u12a5\u1295\u12f0\u121a\u1270\u120b\u1208\u1349 \u1260\u121b\u1325\u1293\u1275) \u12e8\u120d\u121b\u1275 \u1325\u1293\u1275 \u1325\u1293\u1275 \u1241\u120d\u134d \u12d8\u12f4 \u1260\u1230\u12cd \u120d\u1306\u127d \u12cd\u1235\u1325 \u12e8\u121b\u12ed\u127b\u120d \u1290\u12cd \u121d\u12ad\u1295\u12eb\u1271\u121d \u12c8\u122b\u122a \u1290\u12cd \u1361\u1361 \u12e8\u12f6\u12ad\u1270\u122d \u12a2\u126a\u1295 \u12e8\u1225\u122b \u1263\u120d\u12f0\u1228\u1263\u12ce\u127d \u12a8\u1240\u12f5\u121e \u12e8\u1225\u122b \u1263\u120d\u12f0\u1228\u1266\u127b\u1278\u12cd \u130b\u122d \u1270\u12eb\u12ed\u12de \u1260\u1270\u1348\u1325\u122f\u12ca \u121a\u12cd\u1274\u123d\u1295 \u1260\u1230\u12cd \u120d\u1305 \u12a0\u1295\u130e\u120d \u12cd\u1235\u1325 \u1218\u1235\u1218\u122e\u127d\u1295 \u1208\u1218\u1348\u1208\u130d \u120a\u12eb\u1308\u1208\u130d\u1209 \u12a5\u1295\u12f0\u121a\u127d\u1209 \u12a0\u1218\u120d\u12ad\u1270\u12cb\u120d \u1361\u1361 \u1260\u12a0\u1201\u1291 \u12c8\u1245\u1275 \u1260\u122d\u12ab\u1273 \u12e8\u12a0\u1220\u122b\u122d \u12d8\u12f4\u12ce\u127d\u1295 \u1308\u12f0\u1266\u127d \u1260\u1218\u134d\u1273\u1275 \u12ed\u1205\u1295\u1295 \u12d8\u12f4 \u121b\u123b\u123b\u120d \u12a5\u1293 \u12f0\u1228\u1303\u12cd\u1295 \u1208\u121b\u1233\u12f0\u130d \u12d3\u120b\u121b \u12a0\u1208\u12cd \u1361\u1361 \u1260\u1218\u1300\u1218\u122a\u12eb \u1363 \u12e8\u12d8\u122d \u134d\u1208\u130b\u1295 \u12e8\u1260\u1208\u1320 \u12e8\u1290\u1320\u120b \u1234\u120e\u127d \u1325\u1243\u1245\u1295 \u12e8\u12f2 \u12a4\u1295 \u12a4 \u1218\u1320\u1296\u127d \u12e8\u1260\u1208\u1320 \u12a0\u1235\u1270\u121b\u121b\u129d \u121b\u130d\u1208\u120d \u12a5\u1293 \u121b\u1309\u120b\u1275 \u12ed\u1320\u12ed\u1243\u120d\u1362 \u1201\u1208\u1270\u129b \u1363 \u12e8\u1230\u12cd \u12a0\u1295\u130e\u120d \u120d\u121b\u1275 \u12dd\u122d\u12dd\u122d \u130d\u1295\u12db\u1264 \u1260\u123a\u12ce\u127d \u12c8\u12ed\u121d \u1260\u12a0\u1235\u122d \u123a\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u130d\u1208\u1230\u1266\u127d\u1295 \u1215\u12cb\u1233\u1275 \u12a5\u1295\u12f2\u1218\u12d8\u1308\u1265 \u1208\u121b\u12f5\u1228\u130d \u12c8\u132a \u1246\u1323\u1262 \u1218\u1206\u1295 \u12a0\u1208\u1260\u1275\u1362 \u1260\u1218\u1328\u1228\u123b\u121d \u1363 \u1205\u12cb\u1236\u127d\u1295 \u121d\u1295 \u12eb\u1205\u1209 \u121d\u1295 \u12eb\u1205\u120d \u12a5\u1295\u12f0\u121a\u12db\u1218\u12f1 \u1265\u127b \u1233\u12ed\u1206\u1295 \u121d\u1295 \u12d3\u12ed\u1290\u1275 \u1205\u12cb\u1233\u1275 \u121d\u1295 \u12d3\u12ed\u1290\u1275 \u12a5\u1295\u12f0\u1206\u1291 \u1208\u12ed\u1276 \u121b\u12c8\u1245 \u12a0\u1208\u1260\u1275\u1362 \u1276\u1355\u1232\u121d \u12a5\u1290\u12da\u1205\u1295 \u1270\u1348\u1273\u1273\u129d \u127d\u130d\u122e\u127d \u1208\u1218\u134d\u1273\u1275 \u12ed\u1348\u120d\u130b\u120d \u1361\u1361<\/p>\n
\u12e8\u12f6\u12ad\u1270\u122d \u12a2\u126a\u1295 \u12a0\u1240\u122b\u1228\u1265 \u1208\u1201\u1209\u121d \u12e8\u1230\u12cd \u1205\u12cb\u1233\u1275 \u1270\u1348\u133b\u121a \u12e8\u121a\u1206\u1295 \u1232\u1206\u1295 \u1208\u12a0\u12a5\u121d\u122e \u1205\u1218\u121d \u120d\u12e9 \u1275\u12a9\u1228\u1275 \u12e8\u121a\u1235\u1265 \u1290\u12cd \u1361\u1361 \u12a0\u1295\u12f5 \u130a\u12dc \u1324\u1293\u121b \u12e8\u12a0\u1295\u130e\u120d \u12ad\u134d\u120d \u12a8\u1270\u1290\u12f0\u1348 \u1363 \u12a5\u1295\u12f0 \u12a6\u1272\u12dd\u121d \u12a5\u1293 \u1235\u12aa\u12de\u1348\u122a\u1295\u12eb \u12eb\u1209 \u1260\u120d\u121b\u1275 \u12cd\u1235\u1325 \u120a\u12a8\u1230\u1271 \u12a8\u121a\u127d\u1209\u1275 \u12e8\u1270\u1208\u12eb\u12e9 \u127d\u130d\u122e\u127d \u130b\u122d \u12e8\u12a0\u1295\u130e\u120d \u12a5\u12f5\u1308\u1275 \u12a5\u1295\u12f4\u1275 \u12a5\u1295\u12f0\u121a\u1208\u12eb\u12ed \u1208\u1218\u1218\u120d\u12a8\u1275 \u12a5\u1295\u12f0 \u1218\u1230\u1228\u1273\u12ca \u1218\u1230\u1228\u1275 \u1206\u1290\u12cd \u120a\u12eb\u1308\u1208\u130d\u1209 \u12ed\u127d\u120b\u1209\u1362<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\tIaroslav '\u12a0\u120c\u12ad\u1235' Savtchouk, Ph.D.<\/a>\u1363 \u1228\u12f3\u1275 \u1355\u122e\u134c\u1230\u122d \u1363 \u12e8\u1263\u12ee\u121c\u12f2\u12ab\u120d \u1233\u12ed\u1295\u1235 \u12f2\u1353\u122d\u1275\u1218\u1295\u1275 \u1363 \u121b\u122d\u12ad\u1272 \u12e9\u1292\u1268\u122d\u1232\u1272 \u1361\u1361<\/strong><\/p>\n\u1260\u1230\u12d3\u1275 \u1260\u1270\u1230\u12e8\u1218 \u1263\u1208 \u12a0\u122b\u1275 \u121b\u12a5\u12d8\u1295 \u1235\u1274\u122a\u1236\u1235\u12ae\u1355 \u12a0\u121b\u12ab\u129d\u1290\u1275 \u201c\u12e8\u12a0\u1295\u130e\u120d \u1325\u122b\u12dd \u1348\u1323\u1295 \u1295\u12f5\u134d \u121d\u1235\u120d\u1295\u201d<\/strong> <\/em><\/p>\n\u12d8\u1218\u1293\u12ca \u12e8\u12a6\u1355\u1272\u12ab\u120d \u12e8\u12a0\u1295\u130e\u120d \u121d\u1235\u120d \u1274\u12ad\u1292\u12ae\u127d\u1295 \u12e8\u12a0\u1295\u130e\u120d \u1240\u132b\u132d\u1295 \u1295\u1323\u134d \u1208\u1218\u1218\u120d\u12a8\u1275 \u12eb\u1235\u127d\u120b\u1278\u12cb\u120d \u1363 \u1290\u1308\u122d \u130d\u1295 \u12a5\u1295\u12f0 \u1263\u1208 \u12e8\u12a0\u1295\u130e\u120d \u1235\u134b\u1275 \u12eb\u1209 \u1263\u1208 3-\u120d\u12ac\u1275 \u1235\u134b\u1275 \u12cd\u1235\u1325 \u1260\u122d\u12ab\u1273 \u12e8\u12a0\u1295\u130e\u120d \u12a5\u1295\u1245\u1235\u1243\u1234\u12ce\u127d\u1295 \u1218\u1245\u1228\u133d \u12a0\u1235\u12f0\u1295\u130b\u132d \u1206\u1297\u120d \u1361\u1361 \u12f6 \/ \u122d \u1233v\u1275\u1279\u12ad \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1270\u1218\u122b\u121b\u122a\u12ce\u1279 \u1260\u12a0\u1295\u130e\u120d \u12c8\u1208\u120d \u120b\u12ed \u1265\u127b \u1233\u12ed\u1206\u1295 \u12a8\u1218\u127c\u12cd\u121d \u130a\u12dc \u1260\u1260\u1208\u1320 \u1325\u120d\u1245 \u12a5\u1293 \u130a\u12dc\u12eb\u12ca \u1325\u122b\u1275 \u120b\u12ed \u121d\u1295 \u12a5\u12e8\u1270\u1348\u1320\u1228 \u12a5\u1295\u12f3\u1208 \u12a5\u1295\u12f2\u1218\u1208\u12a8\u1271 \u12e8\u121a\u12eb\u1235\u127d\u120d \u12d8\u12f4\u1295 \u12a0\u12d8\u130b\u1305\u1270\u12cb\u120d \u1361\u1361<\/p>\n
\u12cb\u1293\u12cd \u1202\u12f0\u1275 - \u1263\u1208 \u1201\u1208\u1275 \u134e\u12ad\u1235 \u121b\u12ed\u12ad\u122e\u1235\u12ae\u1355 - \u12e8\u120b\u1265\u122b\u1276\u122a \u12a5\u1295\u1235\u1233\u1275 \u1260\u1304\u1294\u1272\u12ab\u12ca \u12e8\u1270\u1235\u1270\u12ab\u12a8\u1209 \u12e8\u12a0\u1295\u130e\u120d \u1234\u120e\u127d \u12cd\u1235\u1325 \u134d\u120e\u1228\u1230\u1295\u1275 \u1260\u1218\u1348\u1208\u130d \u12e8\u12a0\u1295\u130e\u120d\u1295 \u12a5\u1295\u1245\u1235\u1243\u1234 \u12eb\u1290\u1233\u120d\u1362 \u1260\u12a0\u1295\u12f5 \u1290\u1320\u120b \u120c\u12d8\u122d \u1363 \u1325\u120d\u1240\u1275 \u1218\u1228\u1303 \u1260\u1323\u121d \u1260\u1240\u1235\u1273 \u12ed\u1218\u12d8\u1308\u1263\u120d \u1361\u1361 \u1260\u1201\u1208\u1275 \u12e8\u1328\u1228\u122d \u1328\u1228\u122e\u127d \u1363 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u1218\u1220\u1228\u1273\u12ca\u1290\u1275 \u12e8\u1262\u1295\u130e \u12d5\u12ed\u1273\u1295 \u12eb\u1308\u129b\u1209 - \u1245\u122d\u1261\u1295 \u12a5\u1293 \u1245\u122d\u1261\u1295 \u121b\u12e8\u1275 \u12ed\u127d\u120b\u1209 \u1363 \u130d\u1295 \u121d\u1295\u121d \u1290\u1308\u122d \u12e8\u121b\u12ed\u1273\u12ed\u1263\u1278\u12cd \u12e8\u12a5\u12ed\u1273 \u201c\u1325\u120b\u12ce\u127d\u201d \u12a0\u1209 (\u1208\u121d\u1233\u120c \u1363 \u12a0\u1295\u12f5 \u1230\u12cd \u12e8\u127c\u12dd \u1230\u120c\u12f3 \u1320\u122d\u12dd \u1232\u1218\u1208\u12a8\u1275 \u1363 \u12a0\u1295\u12f3\u1295\u12f5 \u1241\u122d\u1325\u122b\u132e\u127d \u1245\u122d\u1265 \u1260\u1206\u1291 \u1241\u122d\u1325\u122b\u132e\u127d \u120a\u1273\u1308\u12f5 \u12ed\u127d\u120b\u120d \u1361\u1361) \u12f6 \/ \u122d \u1233\u126d\u1275\u1279\u12ad \u1201\u1208\u1275 \u12b3\u1236\u127d\u1295\u1293 \u1328\u1228\u122d \u12a5\u12ed\u1273\u12ce\u127d\u1295 \u1260\u121a\u12eb\u1235\u1308\u129d \u12a5\u1293 \u12d3\u12ed\u1290 \u1235\u12cd\u122d \u1266\u1273\u12ce\u127d\u1295 \u1260\u12a8\u134d\u1270\u129b \u1201\u1294\u1273 \u1260\u1218\u1240\u1290\u1235 \u12ed\u1205\u1295\u1295 \u127d\u130d\u122d \u12ed\u1348\u1273\u120d \u1361\u1361 \u12a5\u1231 \u12f0\u130d\u121e \u12e8\u1215\u130d \u1218\u1235\u1218\u122e\u1279\u1295 \u12e8\u130a\u12dc \u1245\u12f0\u121d \u1270\u12a8\u1270\u120d \u12a5\u12e8\u1270\u12a8\u1270\u1208 \u1290\u12cd - \u1260\u134d\u1325\u1290\u1275 \u12e8\u121a\u12eb\u1290\u1243\u1243 - \u1235\u1208\u12da\u1205 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u12e8\u1275\u129b\u12cd\u1295 \u12a5\u1295\u1245\u1235\u1243\u1234 \u12e8\u1275\u129b\u12cd \u1263\u1208 -3-\u120d \u1236\u1235\u1275-\u120d\u12ac\u1275 \u121e\u12f4\u120d \u1208\u1218\u1308\u1295\u1263\u1275 \u12a0\u1235\u1348\u120b\u130a \u12a5\u1295\u12f0\u1206\u1290 \u12eb\u12cd\u1243\u1209\u1362<\/p>\n
\u12e8\u12f6 \/ \u122d \u1233v\u1275\u1279\u12ad \u1355\u122e\u1300\u12ad\u1275 \u1260\u1218\u1300\u1218\u122a\u12eb \u1235\u122d\u12d3\u1271\u1295 \u1260\u12ae\u121d\u1352\u12e9\u1270\u122d \u121b\u1245\u1228\u1262\u12eb\u12ce\u127d \u12f2\u12db\u12ed\u1295 \u121b\u12f5\u1228\u130d \u12a5\u1293 \u12a8\u12da\u12eb \u1218\u1270\u130d\u1260\u122a\u12eb\u12cd\u1295 \u1260\u1218\u12f3\u134a\u1275 \u121e\u12f4\u120e\u127d \u121b\u1228\u130b\u1308\u1325\u1295 \u12eb\u12ab\u1275\u1273\u120d \u1361\u1361 \u12e8\u12a5\u1231 \u130d\u1265 \u1260\u1328\u1228\u122d \u1328\u1228\u122d \u1260\u1218\u1328\u1218\u122d \u12a5\u1295\u12f2\u1201\u121d \u12c8\u12f0 \u1203\u122d\u12f5\u12cc\u122d \u12a5\u1293 \u1236\u134d\u1275\u12cc\u122e\u127d \u1260\u121a\u12f0\u1228\u1309 \u121b\u123b\u123b\u12eb\u12ce\u127d \u12a0\u121b\u12ab\u12ed\u1290\u1275 \u12a0\u1201\u1295 \u120b\u1208\u1201\u1208\u1275 \u134e\u1295\u1276\u1295 \u121b\u12ed\u12ad\u122e-\u134b\u12ed\u120e\u127d\u1295 \u1208\u121b\u12d8\u1218\u1295 \u12e8\u121a\u12eb\u1235\u127d\u1209 \u1218\u1295\u1308\u12f6\u127d\u1295 \u121b\u12d8\u130b\u1300\u1275 \u1290\u12cd \u1361\u1361<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>
\n\t\n\t\t\n\t\t\t
Andre Berndt<\/strong><\/a>, PhD, Assistant Professor, Department of Bioengineering, University of Washington<\/strong><\/p>\n Massively parallel, high throughput engineering of optogenetic biosensors for neuronal signaling<\/em><\/strong><\/p>\n Florescent, genetically encoded proteins have revolutionized the study of brain cells and neural circuits. By literally lighting up in the presence of specific neural activity, which then can be recorded by microscopes and light fibers in living brains, this tool has unlocked many mysteries and allowed researchers to visualize brain activity and neural pathways. But there has been a bottleneck: Developing and identifying the best sensor for each experiment. These encoded proteins need to react in the presence of only specific stimuli, in some cases might need to be highly sensitive, in other cases may need to fluoresce for a longer period of time, or an experiment may need two sensors to see how multiple neurotransmitters interact.<\/p>\n In the past, each sensor had to be genetically modified, produced, and tested individually. Perhaps only a few dozen or hundred could be compared, and researchers chose the best option from a small sample \u2013 not knowing if there was a better, more precise option available. Dr. Berndt has developed a process for developing and testing very large numbers of optogenetic biosensors simultaneously, aiming for screening more than 10,000 per day and building a massive library of biosensors that can give researchers access to precisely engineered proteins they can use to run ever-more specific experiments.<\/p>\n The technology uses rapid genetic engineering to create large numbers of variants of a biosensor, then places individual variants into a microwell array. The sensors are exposed to neuropeptides \u2013 currently, Dr. Berndt is focusing on ligand-specific opioid sensors \u2013 and optical sensors then read the microarray, detecting the brightness and other variables of each variant, and selecting the best options for further testing. Over the course of 2 years, some 750,000 biosensors will be tested and the process for their screening refined, advancing research into opioid actions in the brain and providing a versatile approach other researchers can use for their experiments.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div> Ruixuan Gao<\/strong><\/a>, Ph.D., Assistant Professor, Department of Chemistry and Department of Biological Sciences, University of Illinois Chicago<\/strong><\/p>\n Sub-10 nm spatial profiling of synaptic proteins and RNA transcripts with high-isotropy expansion microscopy using a highly homogenous hydrogel constructed from tetrahedron-like monomers<\/em><\/strong><\/p>\n To examine things that are very small \u2013 like the neurons and their synapses in the brain \u2013 researchers use powerful microscopes. But there is another approach that can yield impressive results: literally expanding a tissue sample and the cells within it by use of a special swellable hydrogel through a process called expansion microscopy. The hydrogel binds to different molecular components of cells and expands, ideally holding all the component parts in the same relative position to one another, creating a larger and more accessible sample to study \u2013 in principle, similar to writing on a balloon, then inflating it.<\/p>\n However, the current hydrogels used for this process have some drawbacks when it comes to studying minute structures in the brain. The margin of error in holding the relative position of molecules isn\u2019t as precise as desired. New gel that potentially overcomes this issue reacts poorly to the heat used in denaturing and treating tissue samples. And it can limit the use of fluorescing biomarkers. Dr. Gao aims to improve the technology by developing a new type of \u201ctetra-gel\u201d, which is chemically engineered to have a tetrahedron-shaped monomer that is extremely uniform as it expands, resists heat and allows the use of bioluminescent markers. He will also develop chemical linkers, specialized molecules that will bind different molecular components of the sample to the gel. The goal is to have an expanded sample that matches the fidelity of the original to within 10 nanometers, matching the resolution of powerful microscopes.<\/p>\n Dr. Gao\u2019s research has already identified promising compounds with which to develop this tetra-gel. As his lab develops and refines it, he will apply its abilities to the study of, for example, early-onset Parkinson\u2019s Disease affected brains. Studying the exact structure of these brains has been challenging with traditional methods, and the goal is to precisely map synaptic proteins and associated gene transcripts, helping uncover how the early onset PD brain is molecularly structured.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div> Mirna Mihovilovic Skanata<\/strong><\/a>, Ph.D., Assistant Professor, Physics Department, Syracuse University <\/strong><\/p>\n Two-photon tracking technology to read and manipulate neural patterns in freely moving animals <\/em><\/strong><\/p>\n The gold standard for neuroscientists is to be able to record and manipulate what is happening in the brain at a high level of precision, over a large area, while a living animal is behaving freely and naturally. Over the years, technology has allowed researchers to move towards this ideal, but always with some compromises. Often, animals needed to be head-fixed, and\/or have intrusive sensors or optics implanted in their brains, and often high-fidelity recording or manipulation was limited to a relatively small area of the brain, while broad-based recordings and manipulation was less precise.<\/p>\n One of the key challenges is simply the motion and distortion of the brain and neurons in a freely-moving animal. But Dr. Skanata is developing a new two-photon tracking technology that allows her to track multiple individual neurons in a moving animal without any invasive implants, and optically activate or manipulate those neurons. The model used is fruit fly larvae, which are naturally transparent, and the system Dr. Skanata will continue to develop uses two-photon microscopes (which allow very precise targeting) coupled with an ingenious algorithm that can rapidly detect the motion of individual neurons and adjust the position of the subject on a moving stage to keep it centered under the microscope. The system calculates the relative positions of multiple neurons, adjusts for the motion and deformation of the brain during movement, and tracks neural activity across a large area.<\/p>\n When tracking an animal that has been modified so that neurons can be activated when exposed to optical light, the system lets researchers switch on neurons with high precision during natural activity. Importantly, the system Dr. Skanata is developing has the capability to independently control two laser beams, so it can track multiple areas simultaneously, and will even allow tracking activity among individuals, allowing insight into neural activity during group encounters.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div> Timothy Dunn, Ph.D.,<\/a><\/strong> Assistant Professor, Department of Biomedical Engineering, Duke University<\/strong><\/p>\n Multi-scale Three Dimensional Behavioral Quantification in Individuals and Social Groups<\/em><\/strong><\/p>\n Current methods of measuring movement of freely behaving animals have limitations: Highly detailed observations of small movements of an animal (a single digit, for example) require restricted ranges of motion. Studying freely moving behavior in 3D space often means limiting resolution, perhaps only tracking overall position, or relying on an observer\u2019s description. Automatic video tracking in animals typically requires an unnatural, simple environment, and body parts not visible to cameras aren\u2019t tracked accurately. High-resolution Artificial Intelligence (AI) predictions over large three-dimensional spaces using volumetric spatial representation, a technique recently developed to overcome these issues, require massive computing power. Adding multiple animals for social observations introduces additional issues.<\/p>\n As a result, there is poor availability of the most desired data: High-resolution, automatic tracking of animals in 3D space performing natural behaviors, alone or in groups, and quantification of that motion in a standardized format. Dr. Dunn is working on a new approach that aims to bring that ideal closer. Building on learnings from a 3D geometric machine-learning algorithm his team used to greatly improve the accuracy of predictions, Dr. Dunn and his team are now working on adaptive recurrent image sampling (ARIS) that combines images from multiple cameras to build a model that can measure and predict body position on many scales, even when a part (such as an arm or foot) isn\u2019t directly visible.<\/p>\n ARIS selectively improves the resolution of fine-scale body features, and uses predictive modelling based on what it knows of its subject (arrangement and length of limbs, how they are connect, how they move, etc.) \u2013 learned first by parsing enormous amounts of training data from freely-behaving rats and then fine-tuned using training data in other species \u2013 to focus on the portion of space where the body part is likely to be. This uses far less computational power than previous 3D volumetric tools. In his research, Dr. Dunn will implement ARIS and record data at multiple scales, from overall position and posture down to the movement of fine features of the hands, feet, and face. Further research will explore its effectiveness with multiple animals interacting. This ability to measure behavior in a new, more precise way has broad implications for the study of neurological disorders that affect movement, linking brain activity to behavior, and studying social interactions.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div> Jeffrey Kieft, Ph.D.,<\/a><\/strong> Professor, Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine<\/strong><\/p>\n A New Technology to Control the Transcriptome<\/em><\/strong><\/p>\n Messenger RNA, or mRNA, is recognized as a vital player in the life and health of cells. These RNA molecules are the templates to make protein, and are created within cells to carry instructions to the protein-making machinery, then are destroyed by enzymes. The totality of mRNA an organism expresses is called its \u201ctranscriptome.\u201d<\/p>\n Deficiencies in mRNA and non-coding RNA (ncRNA) are linked to certain neurodegenerative and neurodevelopmental disorders. If there is too little of a specific mRNA or ncRNA in the transcriptome, certain cellular functions may be degraded or disabled. Dr. Kieft is exploring a novel way to manage the transcriptome by slowing the decay of mRNA and ncRNA. Knowing that some enzymes that destroy the RNAs essentially \u201cchew\u201d it from one end to the other, Dr. Kieft used his understanding of how RNA molecules are structured and fold on themselves to create an engineered piece of exoribonuclease-resistant RNA (xrRNA) that, when introduced to compatible mRNA or ncRNA, combines and folds to form a \u201cblocking\u201d structure, literally changing the shape of the RNA by inserting a protrusion that stops the enzymes in their tracks.<\/p>\n By slowing the decay of the target mRNA and ncRNA, Dr. Kieft sees the opportunity to manage their abundance within the transcriptome. Engineered xrRNAs could recognize just specific targets, link up with them, and create the protection, so researchers can increase the proportion of the target without changing how much is created. The approach has the advantage of being less disruptive to the host cell than unnaturally boosting mRNA, and the precision with which xrRNA can be engineered offers the potential to target multiple RNAs at once, and possibly even allow fine-tuning by precisely managing the rate of decay. Dr. Kieft sees this application, born of basic science studying RNA, as a potentially powerful research tool for neuroscientists, and perhaps even the foundation for therapies in the more distant future.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div> Suhasa Kodandaramaiah, Ph.D.,<\/a><\/strong> Benjamin Mayhugh Assistant Professor, Department of Mechanical Engineering, University of Minnesota Twin Cities <\/strong><\/p>\n Robot Assisted Brain-Wide Recordings in Freely Behaving Mice <\/em><\/strong><\/p>\n Neuroscientists studying brain activity during behaviors usually have to make a trade-off: They use miniaturized head-mounted neural sensors that are light enough to allow a subject animal to behave freely, but are lower resolution or can\u2019t monitor the whole brain. Or they use more powerful tools, which are far too heavy for subject animals and require other solutions, like immobilization while letting animals move on a treadmill, or even using virtual reality experiences that nonetheless limit the behavior of a subject.<\/p>\n Dr. Kodandaramaiah is tackling the challenge with a robotic cranial exoskeleton that carries the weight of neural recording and monitoring hardware while still allowing the subject (in this case a mouse) to rotate its head in all three degrees: a full 360 degree turn in the yaw (horizontal rotation) axis, and about 50 degrees of motion in the pitch and roll axes, while moving around in an arena. The robot has three jointed arms arranged in a triangular configuration, suspended over the subject and meeting at the point of mounting on the head. Sensors in the mount will detect what motion the mouse is making and direct the robot to enable the motion with as little resistive force as possible, allowing the mouse to turn and move within an arena typically used for neuroscience experiments with all the necessary sensory equipment and wires from the implants supported by the robot.<\/p>\n Taking out the need for miniaturization allows researchers to use whatever state-of-the art hardware is available, meaning a robot can theoretically be upgraded to use the latest technology soon after its introduction. To get to that point, Dr. Kodandaramaiah\u2019s team will go through several steps \u2013 engineering the exoskeleton; engineering the head-stage with its needed sensors plus high-density electrodes and cameras for external observation of eyes, whiskers and more; performing benchtop testing; tuning the robot to the inputs a mouse can deliver; determining how to introduce probes; and finally making live recording. With this mechanical underpinning, Dr. Kodandaramaiah hopes to help researchers get closer to the state where they can make detailed brain-wide neural recordings of freely behaving subjects over long timescales.<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div> \u12a2\u126b \u12f3\u12e8\u122d \u1363 \u1352\u12a4\u127d.\u12f2.<\/a>\u1363 \u1228\u12f3\u1275 \u1355\u122e\u134c\u1230\u122d \u1363 \u12cb\u120b\u1235 \u12a4\u127d \u12ae\u1208\u122d \u12e8\u1263\u12ee\u121c\u12f2\u12ab\u120d \u12a2\u1295\u1302\u1290\u122a\u1295\u130d \u12ad\u134d\u120d \u1363 \u12e8\u1306\u122d\u1302\u12eb \u12e8\u1274\u12ad\u1296\u120e\u1302 \u1270\u124b\u121d \u12a5\u1293 \u12a2\u121a\u122a \u12e9\u1292\u1268\u122d\u1232\u1272<\/strong><\/p>\n "<\/em><\/strong>\u1260\u130a\u12dc\u12cd \u1363 \u1260\u1266\u1273 \u12a5\u1293 \u1260\u1263\u1205\u122a\u12eb\u1275 \u12cd\u1235\u1325 \u1275\u120d\u1245 \u1218\u1320\u1295 \u12eb\u1208\u12cd \u12e8\u1290\u122d\u126d \u12cd\u1202\u1266\u127d\u1295 \u1218\u1228\u1303\u12ce\u127d \u121b\u12c8\u12f3\u12f0\u122d \u201d<\/em><\/strong><\/p>\n \u12e8\u1290\u122d\u126d \u12cd\u1202\u1266\u127d\u1295 \u1260\u1275\u120b\u120d\u1245 \u12e8\u12a0\u12d5\u121d\u122e \u12ad\u134d\u120e\u127d \u120b\u12ed \u12e8\u1218\u1246\u1323\u1320\u122d \u12a5\u1293 \u12e8\u1218\u1218\u12dd\u1308\u1265 \u127d\u120e\u1273 \u12a5\u1305\u130d \u1260\u1323\u121d \u1265\u12d9 \u12e8\u1206\u1291 \u12e8\u12cd\u1202\u1265 \u12cd\u1324\u1276\u127d\u1295 \u12a0\u1235\u1308\u129d\u1277\u120d \u1363 \u12ed\u1205\u121d \u1260\u12d3\u1208\u121d \u120b\u12ed \u121d\u1295 \u12eb\u1205\u120d \u12e8\u1290\u122d\u126d \u1205\u12cb\u1236\u127d \u1218\u1228\u1303\u1295 \u1260\u12a0\u1295\u12f5 \u120b\u12ed \u1208\u121b\u12ab\u1270\u1275 \u12a0\u1265\u1228\u12cd\u1295 \u12a5\u1295\u12f0\u121a\u1230\u1229 \u12e8\u121a\u12eb\u1265\u122b\u122b \u1245\u1326\u127d\u1295 \u1208\u121b\u130d\u1298\u1275 \u12a0\u1235\u127d\u120f\u120d \u1361\u1361 \u1260\u12cd\u1202\u1265 \u1235\u1265\u1235\u1266\u127d \u12cd\u1235\u1325 \u12a0\u1290\u1235\u1270\u129b \u1218\u1320\u1295 \u12eb\u120b\u1278\u12cd \u1235\u122d\u12d3\u1270-\u1325\u1208\u1276\u127d\u1295 \u1260\u121b\u130d\u1298\u1275 \u12a0\u12f3\u12f2\u1235 \u1218\u123b\u123b\u120e\u127d \u1262\u1296\u1229\u1275\u121d \u1363 \u1228\u1305\u121d \u130a\u12dc \u122d\u12dd\u1218\u1275 \u12eb\u120b\u1278\u12cd \u12c8\u12ed\u121d \u1265\u12d9 \u12c8\u12ed\u121d \u1270\u1218\u1233\u1233\u12ed \u12c8\u12ed\u121d \u1270\u1218\u1233\u1233\u12ed \u1225\u122b\u12ce\u127d\u1295 \u12e8\u121a\u1348\u1273\u1270\u1291 \u12e8\u1270\u1208\u12eb\u12e9 \u130d\u1208\u1230\u1266\u127d\u1295 \u1363 \u12c8\u12ed\u121d \u1260\u1260\u123d\u1273 \u130d\u12db\u1276\u127d \u120b\u12ed \u121b\u1290\u1343\u1340\u122d \u12a0\u1201\u1295\u121d \u12a8\u1263\u12f5 \u1290\u12cd \u1361\u1361 \u12e8\u12a0\u1295\u130e\u120d \u12a5\u1295\u1245\u1235\u1243\u1234\u1295 \u1208\u1218\u1208\u12e8\u1275 \u12e8\u121b\u123d\u1295 \u1218\u121b\u122a\u12eb (ML) \u1260\u1218\u1320\u1240\u121d \u12e8\u1270\u121e\u12ad\u122e \u120d\u121d\u121d\u12f5 \u1260\u1265\u12d9 \u1275\u120b\u120d\u1245 \u12e8\u1290\u122d\u126d \u12f3\u1273\u1264\u1276\u127d \u12cd\u1235\u1325 \u1235\u122d\u12d3\u1270-\u1325\u1208\u1276\u127d\u1295 \u1208\u1218\u1208\u12e8\u1275 \u12c8\u12f0 \u12a0\u12f2\u1235 \u1218\u134d\u1275\u1204 \u12a5\u1295\u12f5\u1275\u12c8\u1235\u12f5 \u12a0\u12f5\u122d\u1313\u1273\u120d \u1361\u1361<\/p>\n \u12e8\u12f6 \/ \u122d \u12f6yer \u1225\u122b \u12a5\u1295\u1235\u1233\u12cd \u1270\u129d\u1276 \u1363 \u1270\u129d\u1277\u120d \u1363 \u12a0\u1241\u121e \u1290\u1260\u122d \u12c8\u12ed\u121d \u1260\u1270\u1208\u12eb\u12e9 \u12a5\u1295\u1245\u1235\u1243\u1234\u12ce\u127d \u12c8\u12ed\u121d \u1263\u1205\u122a\u12ce\u127d \u12cd\u1235\u1325 \u12e8\u1270\u1218\u12d8\u1308\u1260 \u1218\u1206\u1291\u1295 \u1208\u1218\u1208\u12e8\u1275 \u12a8\u1270\u1230\u12e8\u1219 \u12e8\u1290\u122d\u126d \u12f3\u1273\u1264\u1276\u127d \u1218\u1228\u1303 \u1208\u121b\u12cd\u1323\u1275 \u12e8\u121b\u123d\u1295 \u1275\u121d\u1205\u122d\u1275 \u1235\u120d\u1270 \u1240\u1218\u122e\u127d\u1295 \u1218\u134d\u1320\u122d \u12eb\u12ab\u1275\u1273\u120d \u1361\u1361 \u12a0\u12f2\u1235 \u12e8\u1218\u1228\u1303 \u12a0\u1230\u1323\u1325 \u1270\u1290\u1233\u123d\u1290\u1275 \u12eb\u1208\u12cd \u12e8\u1202\u1233\u1265 \u1205\u130e\u127d \u12cd\u1202\u1261\u1295 \u12c8\u12f0 \u121b\u1218\u1323\u1320\u1295 \u12a5\u1295\u12f0 \u1218\u1290\u123b \u1290\u1325\u1265 \u12e8\u121a\u1218\u1208\u12a8\u1271\u1275 \u12e8\u1270\u1208\u12eb\u12e9 \u12e8\u12a0\u1295\u130e\u120d \u130d\u12db\u1276\u127d \u12e8\u1218\u1290\u1329 \u12e8\u1290\u122d\u126d \u12a5\u1295\u1245\u1235\u1243\u1234\u1295 \u1208\u121b\u12db\u1218\u12f5 \u1260\u1218\u1348\u1208\u130d \u1260\u1270\u1208\u12e8 \u12e8\u1218\u1228\u1303 \u1235\u1265\u1235\u1266\u127d \u12cd\u1235\u1325 \u1270\u1218\u1233\u1233\u12ed \u1235\u122d\u12d3\u1270-\u1325\u1208\u1276\u127d\u1295 \u1208\u1218\u1208\u12e8\u1275 \u1235\u120d\u1270 \u1240\u1218\u1229\u1295 \u12ed\u1218\u122b\u1209 \u1361\u1361 \u12e8\u1290\u122d\u126d \u12a5\u1295\u1245\u1235\u1243\u1234\u1295 \u121b\u1218\u127b\u1278\u1275 \u12e8\u1290\u122d\u126d \u1225\u122d\u12d3\u1276\u127d \u12a8\u122d\u12d5\u1230-\u1263\u1205\u122a\u12cd \u1201\u1294\u1273 \u12a5\u1293 \u1201\u1294\u1273 \u130b\u122d \u12a5\u1295\u12f4\u1275 \u12a5\u1295\u12f0\u121a\u12db\u1218\u12f1 \u12a5\u1295\u12f2\u1201\u121d \u1219\u1235\u1293\u1295 \u1260\u12f5\u121d\u1345 \u1218\u12a8\u120b\u12a8\u120d\u1295 \u12eb\u1233\u12eb\u120d \u1363 \u12a5\u1293\u121d \u1208\u1260\u1208\u1320 \u1283\u12ed\u1208\u129b \u1275\u1295\u1270\u1293 \u1274\u12ad\u1292\u12ae\u127d \u12c8\u1233\u129d \u12a5\u122d\u121d\u1303 \u12ed\u1230\u1323\u120d \u1361\u1361<\/p>\n \u12e8\u12f6 \/ \u122d \u12f6\u1208\u122d \u1201\u1208\u1270\u129b \u12d3\u120b\u121b \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u1290\u122d\u126d \u1290\u122ds\u127d \u120b\u12ed \u1260\u1290\u122d\u126d \u12a5\u1295\u1245\u1235\u1243\u1234 \u12a0\u1320\u1243\u120b\u12ed \u1208\u12cd\u1326\u127d \u12a5\u1295\u12f4\u1275 \u12a0\u1235\u1270\u12cb\u1345\u12a6 \u12a5\u1295\u12f3\u12f0\u1228\u1309 \u12a5\u1293 \u12e8\u1270\u12c8\u1230\u1291 \u12e8\u12a0\u1295\u130e\u120d \u1201\u1294\u1273\u12ce\u127d\u1295 \u1208\u1218\u1270\u1295\u1260\u12ed \u1325\u1245\u121d \u120b\u12ed \u1218\u12cb\u120d \u12a5\u1295\u12f0\u121a\u127d\u1209 \u12a5\u1295\u12f2\u1308\u1290\u12d8\u1261 \u12ed\u1228\u12f3\u120d \u1361\u1361 \u12e8\u1263\u1205\u122a\u12ed \u120d\u12e9\u1290\u1276\u127d \u12c8\u12f0 \u1270\u1208\u12e9 \u12e8\u1215\u12cb\u1235 \u12d3\u12ed\u1290\u1276\u127d \u1218\u1218\u1208\u123b \u120b\u12ed \u1218\u12cb\u120d \u12a0\u1208\u1218\u127b\u1209\u1295 \u1363 \u12a5\u1293 \u1260\u1218\u1228\u1303 \u124b\u1276\u127d \u12cd\u1235\u1325 \u12e8\u1273\u12e9\u1275 \u120d\u12e9\u1290\u1276\u127d \u1260\u12a5\u12eb\u1295\u12f3\u1295\u12f1 \u12a5\u1295\u1235\u1233 \u120b\u12ed \u120d\u12e9\u1290\u1275\u1295 \u1208\u1218\u1208\u12e8\u1275 \u1325\u1245\u121d \u120b\u12ed \u12a5\u1295\u12f2\u12cd\u1209 \u1325\u1293\u1271 \u12e8\u1260\u1208\u1320 \u121d\u122d\u121d\u122d \u12eb\u12f0\u122d\u130b\u120d \u1361\u1361 \u1275\u120b\u120d\u1245 \u12e8\u1290\u122d\u126d \u12e8\u1290\u122d\u126d \u12f3\u1273\u1264\u1276\u127d\u1295 \u12e8\u1218\u1208\u12e8\u1275 \u12a5\u1293 \u12e8\u121b\u1290\u1343\u1340\u122d \u127d\u120e\u1273 \u12e8\u12a0\u1295\u130e\u120d \u12e8\u1290\u122d\u126d \u1225\u122d\u12d3\u1275\u1295 \u1260\u123d\u1273 \u1260\u12a0\u1295\u130e\u120d \u1218\u1228\u1303 \u1202\u12f0\u1275 \u120b\u12ed \u121d\u1295 \u12eb\u1205\u120d \u12a5\u1295\u12f0\u121a\u130e\u12f3 \u1260\u1218\u1320\u1246\u121d \u1260\u1292\u12cd\u122e\u120e\u1302\u12ab\u120d \u121d\u122d\u121d\u122d \u12cd\u1235\u1325 \u1275\u120d\u1245 \u12cb\u130b \u12ed\u1296\u1228\u12cb\u120d \u1361\u1361<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div> \u122a\u12aa\u12aa \u1219\u1208\u122d \u1363 \u1352\u12a4\u127d.\u12f2.<\/a>\u1363 \u1260\u12a4\u120c\u12ad\u1275\u122a\u12ad \u121d\u1205\u1295\u12f5\u1235\u1293 \u12a5\u1293 \u1260\u12ae\u121d\u1352\u12cd\u1270\u122d \u1233\u12ed\u1295\u1235 \u1228\u12f3\u1275 \u1355\u122e\u134c\u1230\u122d \u1363 \u1260\u12ab\u120a\u134e\u122d\u1292\u12eb \u12e9\u1292\u1268\u122d\u1232\u1272 - \u1260\u122d\u12ad\u120c\u12ed<\/strong><\/p>\n "<\/strong><\/em>\u1260\u123a\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u12e8\u1290\u122d\u126d \u1234\u120e\u127d\u1295 \u1208\u12a6\u1355\u120e\u1300\u1295\u1272\u12ab\u12ca \u1241\u1325\u1325\u122d \u12e8\u121a\u1206\u1295 \u12a8\u134d\u1270\u129b \u134d\u1325\u1290\u1275 \u12eb\u1208\u12cd \u12e8\u1206\u120e\u130d\u122b\u134a \u1218\u1223\u122a\u12eb \u201d<\/em><\/strong><\/p>\n \u12a6\u1355\u1276\u1304\u1294\u1272\u12ad\u1235 - \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u1348\u1208\u1309\u1275 \u130a\u12dc \u12a5\u1295\u12f2\u1220\u1229\u1263\u1278\u12cd \u12c8\u12ed\u121d \u12dd\u121d \u12a5\u1295\u12f2\u120d\u120b\u1278\u12cd \u12e8\u1290\u122d\u126d \u1234\u120e\u127d\u1295 \u1260\u12ad\u1265 \u1265\u122d\u1203\u1295-\u1270\u12ae\u122d \u12a5\u1295\u12f2\u1206\u1291 \u1260\u121b\u12f5\u1228\u130d - \u12a6\u12cd\u1276\u120e\u1302\u12ab\u12ca \u12e8\u1290\u122d\u126d-\u121d\u122d\u121d\u122d \u121d\u122d\u121d\u122d\u1295 \u1240\u12ed\u1228\u12cb\u120d \u1361\u1361 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1265\u122d\u1203\u1295\u1295 \u12c8\u12f0 3 \u12f2 \u1202\u120e\u130d\u122b\u121d \u12a8\u121a\u1240\u12eb\u12ed\u1260\u1275 \u12e8\u1266\u1273 \u1265\u122d\u1203\u1295 \u121e\u12f2\u12eb\u1270\u122e\u127d \u130b\u122d \u12e8\u1270\u1323\u1218\u1229 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u1270\u1293\u1325\u120d \u1260\u12a0\u1295\u12f1 \u1263\u1208 \u1236\u1235\u1275 \u12a0\u1245\u1323\u132b\u12ca \u12ad\u120d\u120d \u12cd\u1235\u1325 \u12e8\u121a\u1308\u1299\u1275\u1295 \u1260\u122d\u12ab\u1273 \u12e8\u1290\u122d\u126d \u1234\u120e\u127d\u1295 \u1260\u130d\u120b\u1278\u12cd \u1218\u1246\u1323\u1320\u122d \u12ed\u127d\u120b\u1209 \u1361\u1361 in vivo<\/em>. \u130d\u1295 \u12a5\u1235\u12a8\u12da\u1205 \u12f5\u1228\u1235 \u1260\u1270\u1348\u1325\u122e \u1260\u1270\u1348\u1325\u122e \u1260\u12a0\u1295\u130e\u120d \u12cd\u1235\u1325 \u1260\u121a\u1308\u1299 \u12e8\u1290\u122d\u126d \u134d\u1325\u1290\u1276\u127d \u120b\u12ed \u12e8\u1290\u122d\u126d \u1234\u120e\u127d\u1295 \u1208\u1218\u1246\u1323\u1320\u122d \u12e8\u121a\u12eb\u1235\u127d\u120d \u12e8\u1206\u120e\u130d\u122b\u134a \u1355\u122e\u1304\u12ad\u1275 \u12e8\u1208\u121d \u1361\u1361<\/p>\n \u12f6\u12ad\u1270\u122d \u1219\u1208\u122d \u12ed\u1205\u1295\u1295 \u127d\u130d\u122d \u1208\u1218\u1245\u1228\u134d \u12e8\u1206\u120e\u130d\u122b\u134a \u1355\u122e\u1304\u12ad\u1275 (\u12f2\u12db\u12ed\u1290\u122d) \u12f2\u12db\u12ed\u1295 \u1260\u121b\u12d8\u130b\u1300\u1275 \u120b\u12ed \u1290\u12cd \u1361\u1361 \u1218\u1223\u122a\u12eb\u12cb \u12e8\u1206\u120e\u130d\u122b\u134a\u12ad \u1265\u122d\u1203\u1295 \u121d\u1235\u120e\u127d\u1295 \u1260\u1234\u12ae\u1295\u12f5 \u1260 10,000 \u12ad\u1348\u134e\u127d (\u12a4\u127d.\u12dc.) \u12cb\u130b\u12ce\u127d\u1295 \u1260\u12e5\u1228\u1275 \u12ed\u120d\u1243\u120d \u1361\u1361 \u1265\u12d9 \u12e8\u12c8\u1245\u1271-\u1275\u12cd\u120d\u12f5 \u1274\u120c\u126a\u12e5\u1296\u127d \u1208\u12a0\u1295\u12f5 \u1230\u12a8\u1295\u12f5 60 \u134d\u122c\u121e\u127d\u1295 \u12eb\u12f5\u1233\u1209 \u1363 \u1208\u121b\u1290\u1343\u1340\u122d \u12a5\u1293 \u1260 500 Hz \u12a8\u134d\u1270\u129b \u1325\u122b\u1275 \u120b\u120b\u1278\u12cd \u12e8\u1206\u120e\u130d\u122b\u134a\u12ad \u1218\u1233\u122a\u12eb\u12ce\u127d \u1260\u120b\u1240 \u12f0\u1228\u1303 \u120b\u12ed \u12ed\u1308\u129b\u1209 \u1361\u1361 \u12ed\u1205 \u12a8\u134d\u1270\u129b \u12e8\u121b\u12f0\u1235 \u12f0\u1228\u1303 \u1260\u1270\u1348\u1325\u122e\u12cd \u12e8\u1290\u122d\u126d \u121d\u120d\u12ad\u1275 \u121d\u120d\u12ad\u1275\u1295 \u1208\u1218\u12ae\u1228\u1305 \u12a0\u1235\u1348\u120b\u130a \u1290\u12cd \u1363 \u12ed\u1205\u121d \u1260\u1230\u12a8\u1295\u12f5 1\/1000 \u129b \u130a\u12dc \u120a\u1348\u1320\u122d \u12e8\u121a\u127d\u120d \u12a5\u122d\u121d\u1303\u12ce\u127d\u1295 (\u12a8\u1245\u12f3\u1234 \u1218\u1320\u1295 \u130b\u122d \u1232\u1290\u1343\u1340\u122d \u12a8 1000 Hz \u130b\u122d \u12a5\u12a9\u120d \u1290\u12cd) \u1361\u1361 \u12a5\u1293 \u1260\u1274\u120c\u126a\u12e5\u1296\u127d \u12cd\u1235\u1325 \u12a8\u134d \u12eb\u1208 \u12cb\u130b\u12ce\u127d \u12a0\u1320\u122d \u12eb\u1208 \u121d\u1235\u120e\u127d\u1295 \u12a5\u1295\u12f0\u121a\u1328\u121d\u1229 \u1201\u1209 \u1363 10,000 \u12a4\u127d \u1204\u12dd hologram \u12e8\u120b\u1240 \u1275\u12ad\u12ad\u1208\u129b\u1290\u1275 \u12ed\u1230\u1323\u120d\u1362<\/p>\n \u1260\u1292\u12cd\u122e\u1274\u12ad\u1296\u120e\u1302\u12cd \u120b\u12ed \u12eb\u1270\u12ae\u1228 \u12a4\u120c\u12ad\u1275\u122a\u12ad \u1218\u1210\u1295\u12f2\u1235 \u1363 \u12f2\u12db\u12ed\u1295 \u1260\u121a\u12eb\u12f0\u122d\u1309\u1260\u1275 \u1363 \u1260\u121a\u121e\u12ad\u1229\u1260\u1275 \u12a5\u1293 \u1218\u1233\u122a\u12eb\u12ce\u1279\u1295 \u134d\u120b\u130e\u1276\u127b\u1278\u12cd\u1295 \u12a5\u1295\u12f2\u12eb\u121f\u120b \u1260\u121a\u12eb\u12f0\u122d\u130d\u1260\u1275 \u130a\u12dc \u12e8\u1290\u122d\u126d \u1210\u12aa\u121e\u127d\u1295 \u1260\u1218\u12f0\u1260\u129b\u1290\u1275 \u12eb\u121b\u12ad\u122b\u120d \u1361\u1361 \u1218\u1223\u122a\u12eb\u12cd \u12e8 3 \u12f2 \u1265\u122d\u1203\u1295 \u1201\u1294\u1273\u12ce\u127d\u1295 \u1208\u1270\u12c8\u1230\u1291 \u12a0\u12ab\u1263\u1262\u12ce\u127d \u12a5\u1293 \u1260\u1325\u1243\u1245\u1295 \u1218\u1235\u1273\u12c8\u1276\u127d \u1260\u12a4\u120c\u12ad\u1275\u122a\u12ad \u12a5\u1295\u1245\u1235\u1243\u1234 \u12a0\u121b\u12ab\u129d\u1290\u1275 \u12c8\u12f0 \u121a\u12eb\u1233\u12e8\u12cd \u121b\u12ed\u12ad\u122e\u121a\u122a\u12e8\u122d \u12f5\u122d\u12f5\u122d \u12ed\u1320\u1240\u121b\u120d \u1363 \u12a8\u12da\u12eb \u1265\u122d\u1203\u1291 \u1260\u1270\u12a8\u1273\u1273\u12ed \u120c\u1295\u1236\u127d \u12a0\u121b\u12ab\u12ed\u1290\u1275 \u12ed\u1308\u1208\u133b\u120d \u1361\u1361 \u1218\u122d\u1203\u130d\u1265\u1229 \u1201\u1208\u1275 \u12f5\u122d\u12f5\u122e\u127d\u1295 \u12eb\u1240\u1348 \u12a5\u1293 \u12eb\u12d8\u130b\u1303\u120d - \u1208\u1219\u12a8\u122b \u12a5\u1293 \u1208\u1345\u1295\u1230-\u1200\u1233\u1265 \u12a0\u1290\u1235 \u12eb\u1208 \u12a0\u12f0\u122b\u12f0\u122d \u1363 \u12a5\u1293 \u1208\u1218\u1208\u12ab\u1275 \u12a5\u1293 \u1208\u1218\u1208\u12ab\u1275 \u12a8\u121a\u12eb\u1235\u1348\u120d\u1309\u1275 \u1270\u1313\u12f3\u129d \u1290\u1302\u12ce\u127d \u12a5\u1293 \u1218\u1246\u1323\u1320\u122a\u12eb\u12ce\u127d \u130b\u122d \u12a0\u1295\u12f5 \u1275\u1295\u123d \u12a0\u12f0\u122b\u12f0\u122d\u1362 \u1260\u1218\u1328\u1228\u123b\u121d \u12e8\u12f6 \/ \u122d \u1219\u1209\u1208 \u1261\u12f5\u1295 \u1261\u12f5\u1295 \u1219\u1209 \u1260\u1219\u1209 \u12e8\u1270\u1235\u1270\u12ab\u12a8\u1208 \u12e8\u1266\u1273 \u1265\u122d\u1203\u1295 \u121e\u12f1\u1270\u122d \u12eb\u1235\u1308\u129b\u120d \u1361\u1361 \u12ed\u1205 \u1218\u1223\u122a\u12eb \u1270\u1218\u122b\u121b\u122a\u12ce\u127d\u1295 \u12e8\u1290\u122d\u126d \u130d\u1295\u1299\u1290\u1276\u127d\u1295 \u12e8\u1218\u1246\u1323\u1320\u122d \u12a5\u1293 \u12e8\u1218\u1348\u1270\u123d \u12eb\u120d\u1270\u1208\u1218\u12f0 \u127d\u120e\u1273 \u12ed\u1230\u1323\u1278\u12cb\u120d \u1270\u1265\u120e \u1270\u1235\u134b \u1270\u12f0\u122d\u1313\u120d \u1361\u1361<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div> \u12ab\u12ed \u12da\u1295, \u1352\u12a4\u127d.<\/a>\u1363 \u1203\u12cb\u122d\u12f5 \u12a5\u1293 \u130d\u12cc\u1295 \u120e\u122a \u12e8\u1263\u12ee\u120e\u1302 \u1355\u122e\u134c\u1230\u122d \u1363 \u12ab\u120a\u134e\u122d\u1292\u12eb \u12e8\u1274\u12ad\u1296\u120e\u1302 \u1270\u124b\u121d \u1232\u1218\u1273<\/strong><\/p>\n "<\/em><\/strong>\u121e\u12f1\u120d \u12a2\u1295\u12db\u12ed\u121d\u1272\u12ad \u1263\u122d\u12ae\u12f2\u1295\u130d \u201d<\/em><\/strong><\/p>\n \u1265\u12d9 \u12e8\u1290\u122d\u126d \u1260\u123d\u1273 \u121d\u122d\u1218\u122b\u12ce\u127d \u12e8\u1215\u12cb\u1235 \u1308\u133d\u1273\u1295 \u12e8\u121a\u12a8\u120b\u12a8\u1209 \u1340\u1228-\u1230\u12cd \u12a5\u1293 \u12e8\u1270\u1240\u1263\u12ee\u127d \u1275\u1295\u1273\u1294 \u12eb\u12ab\u1275\u1273\u1209\u1362 \u12f0\u130d\u121e\u121d \u1235\u1208 \u12e8\u1290\u122d\u126d \u120d\u121b\u1275 \u12a5\u1293 \u1270\u130d\u1263\u122d \u130d\u1295\u12db\u1264 \u1235\u1208 \u12a5\u12cd\u1240\u1275 \u12ed\u1320\u12ed\u1243\u120d in vivo<\/em> \u12e8\u1215\u12cb\u1235 \u12c8\u1208\u120d \u1355\u122e\u1272\u1296\u127d \u1218\u12ab\u12a8\u120d \u130d\u1295\u1299\u1290\u1276\u127d\u1362 \u12a5\u12eb\u1295\u12f3\u1295\u12f1 \u1355\u122e\u1272\u1295 \u12e8\u1270\u1208\u12eb\u12e9 \u1263\u12ee\u12ac\u121a\u12ab\u12ca \u1263\u1205\u122a\u12ce\u127d \u1235\u120b\u1209\u1275 \u1355\u122e\u1272\u1296\u127d\u1295 \u12e8\u121a\u12eb\u12ab\u1275\u1271 \u12a8\u134d\u1270\u129b \u12e8\u121d\u122d\u121d\u122d \u1219\u12a8\u122b\u12ce\u127d \u130a\u12dc\u1295 \u12e8\u121a\u12c8\u1235\u12f5 \u12a5\u1293 \u12cd\u1235\u1265\u1235\u1265 \u1293\u1278\u12cd\u1362 \u12e8\u1290\u122d\u126d \u1233\u12ed\u1295\u1235 \u121d\u122d\u121d\u122d \u12a0\u12f3\u12f2\u1235 \u12d5\u12f5\u120e\u127d\u1295 \u1208\u1218\u12ad\u1348\u1275 \u1208\u121b\u1308\u12dd \u12f6\u12ad\u1270\u122d \u12d8\u12ed\u1295 \u12a5\u1293 \u12e8\u12a5\u1231 \u1261\u12f5\u1295 \u12e8\u1270\u1208\u12eb\u12e9 \u1355\u122e\u1272\u1296\u127d\u1295 \u201c\u1263\u122d\u12ae\u12f5\u201d \u12e8\u1270\u1208\u12eb\u12e9 \u1355\u122e\u1272\u1296\u127d\u1295 (modcode) \u1218\u1295\u1308\u12f5 \u1260\u1218\u134d\u1320\u122d \u1270\u1218\u122b\u121b\u122a\u12ce\u127d\u1295 \u1270\u1208\u12cb\u12cb\u132d \u12e8\u1218\u1233\u122a\u12eb \u12aa\u1235 \u12ed\u1230\u1323\u1209 \u1361\u1361<\/p>\n \u1263\u122d\u12ae\u12f2\u1295\u130d \u1260\u1240\u120b\u120d \u1245\u122d\u1345 \u12e8\u1304\u1294\u1272\u12ad \u12a0\u1218\u120d\u12ab\u127e\u127d\u1295 \u12c8\u12f0 \u121e\u1208\u12aa\u12cd\u120e\u127d \u12cd\u1235\u1325 \u121b\u1235\u1308\u1263\u1275 \u12a5\u1293 \u12a8\u12db\u121d \u1219\u12a8\u122b\u12cd \u1260\u128b\u120b \u12e8\u1275\u129e\u1279 \u121e\u1208\u12aa\u12cd\u120e\u127d \u1260\u12a0\u1295\u12f5 \u120b\u12ed \u12a5\u1295\u12f0\u1270\u1320\u1246\u1219 \u1208\u121b\u12c8\u1245 \u12a5\u1290\u12da\u1205\u1295 \u12a0\u1218\u120d\u12ab\u127e\u127d \u1218\u1348\u1208\u130d\u1295 \u12eb\u12ab\u1275\u1273\u120d\u1362 \u12a8\u1273\u120b\u1245 \u1235\u12ac\u1275 \u130b\u122d \u12a8\u1292\u12cd\u12ad\u120a\u12ad \u12a0\u1232\u12f5 \u130b\u122d \u1325\u1245\u121d \u120b\u12ed \u12cd\u120f\u120d\u1362 \u1355\u122e\u1272\u1296\u127d \u12ed\u1260\u120d\u1325 \u12e8\u1270\u12c8\u1233\u1230\u1261 \u1293\u1278\u12cd \u1363 \u130d\u1295 \u1265\u12d9 \u130a\u12dc \u12e8\u1355\u122e\u1272\u1295 \u1270\u130d\u1263\u122d\u1295 \u12e8\u121a\u1240\u12ed\u122d \u12ac\u121a\u12ab\u12ca \u12a0\u124b\u122b\u132d \u1218\u1295\u1308\u12f5 \u1233\u12ed\u1296\u122b\u1278\u12cd \u1208\u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u123a\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u12e8\u134d\u120b\u130e\u1275 \u1355\u122e\u1272\u1296\u127d\u1295 \u1208\u1218\u1208\u12eb\u12e8\u1275 \u12e8\u121a\u12eb\u1235\u127d\u120d \u1218\u1295\u1308\u12f5 \u12a0\u120d\u1290\u1260\u1228\u121d\u1362 \u12f6 \/ \u122d \u12da\u1295 \u12a8 \u201cHUH-domain\u201d \u12a2\u1295\u12db\u12ed\u121e\u127d \u130b\u122d \u12e8\u1270\u1246\u122b\u1299 \u12a8\u134d\u1270\u129b-\u12e8\u130d\u1295\u1299\u1290\u1275 \u1355\u122e\u1272\u1295 \u121b\u12eb\u12eb\u12e3 \u121e\u1301\u120e\u127d\u1295 \u12e8\u12eb\u12d9 \u12e8\u1305\u121d\u120b \u1355\u122e\u1272\u1296\u127d\u1295 \u1260\u1218\u1320\u1240\u121d \u12ed\u1205\u1295\u1295 \u1270\u130d\u12f3\u122e\u1275 \u12a5\u12eb\u1238\u1290\u1349 \u1290\u12cd \u1361\u1361 \u1270\u1323\u1263\u1242 \u121e\u1301\u120e\u1279 \u1263\u122d\u12ae\u12ae\u12ae\u127d\u1295 \u12a8\u1340\u1228-\u1270\u1215\u12cb\u1235\u12eb\u1295 \u1363 \u12a8\u1262\u12ee\u1273\u12ed\u121a\u12ad \u1355\u122e\u1272\u1296\u127d \u12a5\u1293 \u1355\u122e\u1272\u1296\u127d \u130b\u122d \u12e8\u1270\u1233\u1230\u1229 \u12e8\u1218\u12eb\u12e3 \u1218\u1208\u12eb\u12ce\u127d \u130b\u122d \u12a5\u1295\u12f2\u1323\u1260\u1241 \u12eb\u1235\u127d\u120b\u1278\u12cb\u120d \u1361\u1361 \u12ed\u1205 \u1208\u12a0\u1265\u12db\u129b\u12ce\u1279 \u12e8\u1290\u122d\u126d \u1210\u12aa\u121e\u127d \u12a8\u134d\u1270\u129b \u134d\u120b\u130e\u1275 \u12eb\u120b\u1278\u12cd \u1355\u122e\u1272\u1296\u127d \u1218\u12f3\u1228\u123b\u1295 \u12ed\u1230\u1323\u120d \u1361\u1361 \u1260\u1270\u1328\u121b\u122a\u121d \u1218\u122d\u1203\u130d\u1265\u1229 \u1293\u1296\u12a0\u134d\u120a\u12ab\u120d \u1235\u12ab\u1290\u122e\u127d\u1295 \u12e8\u121a\u12ed\u12d9 60 \u12e8\u121b\u12eb\u12eb\u12e3 \u1290\u1325\u1266\u127d\u1295 \u1260\u1270\u1218\u1233\u1233\u12ed \u130a\u12dc \u12a8\u1263\u122d\u12ae\u12f5 \u12a5\u1293 \u12a8\u134d\u120b\u130e\u1275 \u1355\u122e\u1272\u1296\u127d \u130b\u122d \u121b\u12eb\u12eb\u12dd \u12ed\u127d\u120b\u120d \u1361\u1361 \u12a5\u1290\u12da\u1205 \u1245\u122d\u134a\u1276\u127d \u12e8\u130d\u1295\u1299\u1290\u1276\u127d\u1295 \u121d\u120d\u12a8\u1273 \u12eb\u123b\u123d\u120b\u1209 - \u1260\u12a5\u12eb\u1295\u12f3\u1295\u12f1 \u1218\u12cb\u1245\u122d \u120b\u12ed \u12eb\u1209 \u1260\u122d\u12ab\u1273 \u1355\u122e\u1272\u1296\u127d \u1232\u1308\u1293\u1299 \u12f0\u12ab\u121b \u130d\u1295\u1299\u1290\u1276\u127d \u1320\u1295\u12ab\u122b \u12ed\u1206\u1293\u1209\u1362<\/p>\n \u12e8\u12f6\u12ad\u1270\u122d \u12dc\u1295 \u1355\u122e\u1300\u12ad\u1275 \u1355\u122e\u1272\u1296\u127d\u1295 \u12a5\u1293 \u1218\u1228\u1303\u12ce\u127d\u1295 \u12e8\u121a\u12eb\u1240\u122d\u1261 \u1260\u122d\u12ab\u1273 \u12a8\u134d\u1270\u129b-\u1290\u1320\u120b-\u1290\u1320\u120b-\u1290\u1320\u120b-\u1234\u120d \u1245\u12f0\u121d-\u1270\u12a8\u1270\u120d \u1219\u12a8\u122b\u12ce\u127d\u1295 \u1260\u121b\u12ab\u1204\u12f5 \u120b\u12ed \u12e8\u1270\u1233\u1270\u1349 \u1355\u122e\u1276\u12ae\u120e\u127d\u1295 \u12a5\u1293 \u1202\u12f0\u1276\u127d\u1295 \u121b\u12d8\u130b\u1300\u1275 \u12ed\u1300\u121d\u122b\u120d \u1361\u1361 \u12a5\u1290\u12da\u1205 \u1260\u12a0\u1295\u12f5 \u1234\u120d \u120b\u12ed \u12e8\u1270\u12c8\u1230\u1291 \u12e8\u1270\u12c8\u1230\u1291 \u1270\u1240\u1263\u12ee\u127d \u1270\u1240\u1263\u12ee\u127d \u12a0\u1308\u120b\u1208\u1345\u1295 \u1208\u1218\u1218\u120d\u12a8\u1275 \u1363 \u1208\u12a0\u1295\u12f3\u1295\u12f5 \u1355\u122e\u1272\u1296\u127d \u1232\u130b\u1208\u1321 \u1260\u1234\u120e\u127d \u120b\u12ed \u12e8\u121a\u12f0\u1228\u1309 \u1208\u12cd\u1326\u127d\u1295 \u1208\u1218\u1218\u120d\u12a8\u1275 \u1363 \u1260\u12a0\u1295\u130e\u120d \u1215\u1265\u1228 \u1215\u12cb\u1233\u1275 \u12cd\u1235\u1325 \u1265\u12d9 \u12a0\u1295\u1272\u1302\u1296\u127d\u1295 \u1208\u1218\u1308\u1218\u1275 \u1363 \u12e8\u1355\u122e\u1272\u1296\u127d \u1265\u12db\u1275 \u1218\u1235\u1270\u130b\u1265\u122d\u1295 \u1208\u121b\u12e8\u1275 \u12a5\u1293 \u1208 \u201c\u12c8\u120b\u1305 \u12a0\u120d\u1263\u201d \u1355\u122e\u1272\u1296\u127d \u1270\u1240\u1263\u12ee\u127d\u1295 \u1218\u1208\u12e8\u1275 \u1361\u1361 \u1263\u1208\u121e\u12eb\u1290\u1271 \u1363 \u1240\u120b\u120d\u1290\u1271 \u12a5\u1293 \u1260\u12a0\u1295\u12f5 \u130a\u12dc \u1260\u122d\u12ab\u1273 \u1355\u122e\u1272\u1296\u127d \u12a5\u1295\u12f2\u1230\u1229 \u12e8\u1218\u134d\u1240\u12f5 \u127d\u120e\u1273\u12cd \u121d\u1235\u130b\u1293 \u12ed\u130d\u1263\u12cd\u1293 \u12f6 \/ \u122d \u12da\u1295 \u12e8\u12a5\u1231 \u12e8\u1218\u122d\u1308\u132b \u1235\u122d\u12d3\u1271 \u12a5\u1290\u12da\u1205\u1295 \u12a5\u1293 \u120c\u120e\u127d \u1260\u122d\u12ab\u1273 \u12e8\u1290\u122d\u126d \u1205\u120a\u1293 \u1219\u12a8\u122b\u12ce\u127d\u1295 \u12eb\u134b\u1325\u1293\u120d \u12a5\u1293 \u12eb\u134b\u1325\u1293\u120d \u1361\u1361<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div> \u1308\u122d\u120d \u12a2\u126d\u122e\u1295 \u1363 \u12a4\u121d.\u12a0\u122d. \u1363 \u1352. \u12f2. \u1363<\/a> \u1228\u12f3\u1275 \u1355\u122e\u134c\u1230\u122d \u1363 \u12e8\u1230\u12cd \u12d8\u1228-\u1218\u120d (\u1305\u1295) \u12a5\u1293 \u1302\u12a6\u120e\u1302\u12ad\u1235 \u121b\u12d5\u12a8\u120d \u12e8\u1292\u12cd \u12ee\u122d\u12ad \u12e9\u1292\u1268\u122d\u1232\u1272 \u120b\u1295\u130d\u1276\u1295 \u1324\u1293<\/strong><\/p>\n \u201c\u1275\u12d5\u12ed\u1295\u1275-\u12a8\u134d\u1270\u129b \u12e8\u12a0\u12a5\u121d\u122e \u12d8\u1228-\u1218\u120d\u1218\u12f5\u1295 \u1208\u1230\u1265\u12a0\u12ca \u134d\u1230\u1275 \u12a0\u1230\u1323\u1325 \u1290\u1320\u120b-\u1234\u120d \u1263\u1208\u1265\u12d9-\u1274\u12ad\u1296\u120e\u1302 \u1274\u12ad\u1296\u120e\u1302\u201d<\/em><\/strong><\/p>\n \u12a0\u1295\u12f5 \u12a5\u12eb\u1295\u12f3\u1295\u12f1 \u134d\u1321\u122d \u12a0\u1295\u12f5 \u1290\u1320\u120b \u12f2\u1293 \u201c\u1218\u1218\u122a\u12eb\u12ce\u127d\u201d \u12eb\u1208\u12cd \u12a0\u1295\u12f5 \u1205\u12cb\u1235 \u12e8\u121a\u1300\u121d\u122d\u1260\u1275 \u12a0\u1295\u12f5 \u1215\u12cb\u1235 \u12a5\u1295\u12f0\u1206\u1290 \u12e8\u121a\u1273\u12c8\u1245 \u1290\u1308\u122d \u130d\u1295 \u12a0\u1295\u12f5 \u1205\u12cb\u1235 \u1260\u123a\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u1260\u1262\u120a\u12ee\u1295 \u12e8\u121a\u1246\u1320\u1229 \u1205\u12cb\u1233\u1275\u1295 \u1328\u121d\u122e - \u12a0\u1201\u1295\u121d \u1260\u12a0\u12a5\u121d\u122e \u12cd\u1235\u1325 \u1308\u1293 \u12a0\u120d\u1273\u12c8\u1240\u121d \u1361\u1361 \u12e8\u12f6\u12ad\u1270\u122d \u12a2\u126a\u1295\u1292 \u121d\u122d\u121d\u122d \u12e8\u1273\u1218\u1240 \u1215\u12cb\u1233\u1275 \u1260\u1230\u12cd \u12a0\u1295\u130e\u120d \u12cd\u1235\u1325 \u120b\u1209\u1275 \u1260\u1218\u1276\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u12e8\u130e\u120d\u121b\u1233 \u1234\u120e\u127d \u12d3\u12ed\u1290\u1276\u127d \u12e8\u1275\u129b\u12cd\u1295 \u12e8\u12d8\u122d \u1205\u12cb\u1235 \u1355\u122e\u1272\u1296\u127d \u12a5\u1295\u12f0\u121a\u1230\u1321 \u1260\u1218\u130d\u1208\u133d \u12ed\u1205\u1295\u1295 \u1202\u12f0\u1275 \u12e8\u121a\u12eb\u1265\u122b\u122b \u201c\u1275\u122a\u1355\u1232\u201d \u12e8\u1270\u1263\u1208 \u1274\u12ad\u1296\u120e\u1302\u1295 \u121b\u12f3\u1260\u122d \u1290\u12cd \u1361\u1361<\/p>\n \u1274\u12ad\u1296\u120e\u1302\u12cd \u12e8\u1230\u12ce\u127d\u1295 \u12e8\u12a0\u1295\u130e\u120d \u12a5\u12f5\u1308\u1275 \u12e8\u121a\u12eb\u1320\u1291 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u12e8\u121a\u12eb\u130b\u1325\u121f\u1278\u12cd\u1295 \u1241\u120d\u134d \u1241\u120d\u134d \u1309\u12f3\u12ee\u127d \u120a\u1348\u1273 \u12ed\u127d\u120b\u120d \u1361\u1361 \u1218\u1235\u1218\u122e\u127d\u1295 \u1260\u1218\u1348\u1208\u130d (\u12d8\u12f4\u12ce\u127d\u1295 \u12c8\u12f0 \u12eb\u120d\u1260\u1241 \u12a5\u1295\u1235\u1233\u1275 \u1215\u12cb\u1233\u1275 \u12cd\u1235\u1325 \u1260\u121b\u1235\u1308\u1263\u1275 \u12a5\u1293 \u12a8\u12da\u12eb \u12a0\u1218\u120d\u12ab\u127e\u127d \u12c8\u12f0 \u12d8\u122e\u127b\u1278\u12cd \u12a5\u1295\u12f4\u1275 \u12a5\u1295\u12f0\u121a\u1270\u120b\u1208\u1349 \u1260\u121b\u1325\u1293\u1275) \u12e8\u120d\u121b\u1275 \u1325\u1293\u1275 \u1325\u1293\u1275 \u1241\u120d\u134d \u12d8\u12f4 \u1260\u1230\u12cd \u120d\u1306\u127d \u12cd\u1235\u1325 \u12e8\u121b\u12ed\u127b\u120d \u1290\u12cd \u121d\u12ad\u1295\u12eb\u1271\u121d \u12c8\u122b\u122a \u1290\u12cd \u1361\u1361 \u12e8\u12f6\u12ad\u1270\u122d \u12a2\u126a\u1295 \u12e8\u1225\u122b \u1263\u120d\u12f0\u1228\u1263\u12ce\u127d \u12a8\u1240\u12f5\u121e \u12e8\u1225\u122b \u1263\u120d\u12f0\u1228\u1266\u127b\u1278\u12cd \u130b\u122d \u1270\u12eb\u12ed\u12de \u1260\u1270\u1348\u1325\u122f\u12ca \u121a\u12cd\u1274\u123d\u1295 \u1260\u1230\u12cd \u120d\u1305 \u12a0\u1295\u130e\u120d \u12cd\u1235\u1325 \u1218\u1235\u1218\u122e\u127d\u1295 \u1208\u1218\u1348\u1208\u130d \u120a\u12eb\u1308\u1208\u130d\u1209 \u12a5\u1295\u12f0\u121a\u127d\u1209 \u12a0\u1218\u120d\u12ad\u1270\u12cb\u120d \u1361\u1361 \u1260\u12a0\u1201\u1291 \u12c8\u1245\u1275 \u1260\u122d\u12ab\u1273 \u12e8\u12a0\u1220\u122b\u122d \u12d8\u12f4\u12ce\u127d\u1295 \u1308\u12f0\u1266\u127d \u1260\u1218\u134d\u1273\u1275 \u12ed\u1205\u1295\u1295 \u12d8\u12f4 \u121b\u123b\u123b\u120d \u12a5\u1293 \u12f0\u1228\u1303\u12cd\u1295 \u1208\u121b\u1233\u12f0\u130d \u12d3\u120b\u121b \u12a0\u1208\u12cd \u1361\u1361 \u1260\u1218\u1300\u1218\u122a\u12eb \u1363 \u12e8\u12d8\u122d \u134d\u1208\u130b\u1295 \u12e8\u1260\u1208\u1320 \u12e8\u1290\u1320\u120b \u1234\u120e\u127d \u1325\u1243\u1245\u1295 \u12e8\u12f2 \u12a4\u1295 \u12a4 \u1218\u1320\u1296\u127d \u12e8\u1260\u1208\u1320 \u12a0\u1235\u1270\u121b\u121b\u129d \u121b\u130d\u1208\u120d \u12a5\u1293 \u121b\u1309\u120b\u1275 \u12ed\u1320\u12ed\u1243\u120d\u1362 \u1201\u1208\u1270\u129b \u1363 \u12e8\u1230\u12cd \u12a0\u1295\u130e\u120d \u120d\u121b\u1275 \u12dd\u122d\u12dd\u122d \u130d\u1295\u12db\u1264 \u1260\u123a\u12ce\u127d \u12c8\u12ed\u121d \u1260\u12a0\u1235\u122d \u123a\u12ce\u127d \u12e8\u121a\u1246\u1320\u1229 \u130d\u1208\u1230\u1266\u127d\u1295 \u1215\u12cb\u1233\u1275 \u12a5\u1295\u12f2\u1218\u12d8\u1308\u1265 \u1208\u121b\u12f5\u1228\u130d \u12c8\u132a \u1246\u1323\u1262 \u1218\u1206\u1295 \u12a0\u1208\u1260\u1275\u1362 \u1260\u1218\u1328\u1228\u123b\u121d \u1363 \u1205\u12cb\u1236\u127d\u1295 \u121d\u1295 \u12eb\u1205\u1209 \u121d\u1295 \u12eb\u1205\u120d \u12a5\u1295\u12f0\u121a\u12db\u1218\u12f1 \u1265\u127b \u1233\u12ed\u1206\u1295 \u121d\u1295 \u12d3\u12ed\u1290\u1275 \u1205\u12cb\u1233\u1275 \u121d\u1295 \u12d3\u12ed\u1290\u1275 \u12a5\u1295\u12f0\u1206\u1291 \u1208\u12ed\u1276 \u121b\u12c8\u1245 \u12a0\u1208\u1260\u1275\u1362 \u1276\u1355\u1232\u121d \u12a5\u1290\u12da\u1205\u1295 \u1270\u1348\u1273\u1273\u129d \u127d\u130d\u122e\u127d \u1208\u1218\u134d\u1273\u1275 \u12ed\u1348\u120d\u130b\u120d \u1361\u1361<\/p>\n \u12e8\u12f6\u12ad\u1270\u122d \u12a2\u126a\u1295 \u12a0\u1240\u122b\u1228\u1265 \u1208\u1201\u1209\u121d \u12e8\u1230\u12cd \u1205\u12cb\u1233\u1275 \u1270\u1348\u133b\u121a \u12e8\u121a\u1206\u1295 \u1232\u1206\u1295 \u1208\u12a0\u12a5\u121d\u122e \u1205\u1218\u121d \u120d\u12e9 \u1275\u12a9\u1228\u1275 \u12e8\u121a\u1235\u1265 \u1290\u12cd \u1361\u1361 \u12a0\u1295\u12f5 \u130a\u12dc \u1324\u1293\u121b \u12e8\u12a0\u1295\u130e\u120d \u12ad\u134d\u120d \u12a8\u1270\u1290\u12f0\u1348 \u1363 \u12a5\u1295\u12f0 \u12a6\u1272\u12dd\u121d \u12a5\u1293 \u1235\u12aa\u12de\u1348\u122a\u1295\u12eb \u12eb\u1209 \u1260\u120d\u121b\u1275 \u12cd\u1235\u1325 \u120a\u12a8\u1230\u1271 \u12a8\u121a\u127d\u1209\u1275 \u12e8\u1270\u1208\u12eb\u12e9 \u127d\u130d\u122e\u127d \u130b\u122d \u12e8\u12a0\u1295\u130e\u120d \u12a5\u12f5\u1308\u1275 \u12a5\u1295\u12f4\u1275 \u12a5\u1295\u12f0\u121a\u1208\u12eb\u12ed \u1208\u1218\u1218\u120d\u12a8\u1275 \u12a5\u1295\u12f0 \u1218\u1230\u1228\u1273\u12ca \u1218\u1230\u1228\u1275 \u1206\u1290\u12cd \u120a\u12eb\u1308\u1208\u130d\u1209 \u12ed\u127d\u120b\u1209\u1362<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div> Iaroslav '\u12a0\u120c\u12ad\u1235' Savtchouk, Ph.D.<\/a>\u1363 \u1228\u12f3\u1275 \u1355\u122e\u134c\u1230\u122d \u1363 \u12e8\u1263\u12ee\u121c\u12f2\u12ab\u120d \u1233\u12ed\u1295\u1235 \u12f2\u1353\u122d\u1275\u1218\u1295\u1275 \u1363 \u121b\u122d\u12ad\u1272 \u12e9\u1292\u1268\u122d\u1232\u1272 \u1361\u1361<\/strong><\/p>\n \u1260\u1230\u12d3\u1275 \u1260\u1270\u1230\u12e8\u1218 \u1263\u1208 \u12a0\u122b\u1275 \u121b\u12a5\u12d8\u1295 \u1235\u1274\u122a\u1236\u1235\u12ae\u1355 \u12a0\u121b\u12ab\u129d\u1290\u1275 \u201c\u12e8\u12a0\u1295\u130e\u120d \u1325\u122b\u12dd \u1348\u1323\u1295 \u1295\u12f5\u134d \u121d\u1235\u120d\u1295\u201d<\/strong> <\/em><\/p>\n \u12d8\u1218\u1293\u12ca \u12e8\u12a6\u1355\u1272\u12ab\u120d \u12e8\u12a0\u1295\u130e\u120d \u121d\u1235\u120d \u1274\u12ad\u1292\u12ae\u127d\u1295 \u12e8\u12a0\u1295\u130e\u120d \u1240\u132b\u132d\u1295 \u1295\u1323\u134d \u1208\u1218\u1218\u120d\u12a8\u1275 \u12eb\u1235\u127d\u120b\u1278\u12cb\u120d \u1363 \u1290\u1308\u122d \u130d\u1295 \u12a5\u1295\u12f0 \u1263\u1208 \u12e8\u12a0\u1295\u130e\u120d \u1235\u134b\u1275 \u12eb\u1209 \u1263\u1208 3-\u120d\u12ac\u1275 \u1235\u134b\u1275 \u12cd\u1235\u1325 \u1260\u122d\u12ab\u1273 \u12e8\u12a0\u1295\u130e\u120d \u12a5\u1295\u1245\u1235\u1243\u1234\u12ce\u127d\u1295 \u1218\u1245\u1228\u133d \u12a0\u1235\u12f0\u1295\u130b\u132d \u1206\u1297\u120d \u1361\u1361 \u12f6 \/ \u122d \u1233v\u1275\u1279\u12ad \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1270\u1218\u122b\u121b\u122a\u12ce\u1279 \u1260\u12a0\u1295\u130e\u120d \u12c8\u1208\u120d \u120b\u12ed \u1265\u127b \u1233\u12ed\u1206\u1295 \u12a8\u1218\u127c\u12cd\u121d \u130a\u12dc \u1260\u1260\u1208\u1320 \u1325\u120d\u1245 \u12a5\u1293 \u130a\u12dc\u12eb\u12ca \u1325\u122b\u1275 \u120b\u12ed \u121d\u1295 \u12a5\u12e8\u1270\u1348\u1320\u1228 \u12a5\u1295\u12f3\u1208 \u12a5\u1295\u12f2\u1218\u1208\u12a8\u1271 \u12e8\u121a\u12eb\u1235\u127d\u120d \u12d8\u12f4\u1295 \u12a0\u12d8\u130b\u1305\u1270\u12cb\u120d \u1361\u1361<\/p>\n \u12cb\u1293\u12cd \u1202\u12f0\u1275 - \u1263\u1208 \u1201\u1208\u1275 \u134e\u12ad\u1235 \u121b\u12ed\u12ad\u122e\u1235\u12ae\u1355 - \u12e8\u120b\u1265\u122b\u1276\u122a \u12a5\u1295\u1235\u1233\u1275 \u1260\u1304\u1294\u1272\u12ab\u12ca \u12e8\u1270\u1235\u1270\u12ab\u12a8\u1209 \u12e8\u12a0\u1295\u130e\u120d \u1234\u120e\u127d \u12cd\u1235\u1325 \u134d\u120e\u1228\u1230\u1295\u1275 \u1260\u1218\u1348\u1208\u130d \u12e8\u12a0\u1295\u130e\u120d\u1295 \u12a5\u1295\u1245\u1235\u1243\u1234 \u12eb\u1290\u1233\u120d\u1362 \u1260\u12a0\u1295\u12f5 \u1290\u1320\u120b \u120c\u12d8\u122d \u1363 \u1325\u120d\u1240\u1275 \u1218\u1228\u1303 \u1260\u1323\u121d \u1260\u1240\u1235\u1273 \u12ed\u1218\u12d8\u1308\u1263\u120d \u1361\u1361 \u1260\u1201\u1208\u1275 \u12e8\u1328\u1228\u122d \u1328\u1228\u122e\u127d \u1363 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u1260\u1218\u1220\u1228\u1273\u12ca\u1290\u1275 \u12e8\u1262\u1295\u130e \u12d5\u12ed\u1273\u1295 \u12eb\u1308\u129b\u1209 - \u1245\u122d\u1261\u1295 \u12a5\u1293 \u1245\u122d\u1261\u1295 \u121b\u12e8\u1275 \u12ed\u127d\u120b\u1209 \u1363 \u130d\u1295 \u121d\u1295\u121d \u1290\u1308\u122d \u12e8\u121b\u12ed\u1273\u12ed\u1263\u1278\u12cd \u12e8\u12a5\u12ed\u1273 \u201c\u1325\u120b\u12ce\u127d\u201d \u12a0\u1209 (\u1208\u121d\u1233\u120c \u1363 \u12a0\u1295\u12f5 \u1230\u12cd \u12e8\u127c\u12dd \u1230\u120c\u12f3 \u1320\u122d\u12dd \u1232\u1218\u1208\u12a8\u1275 \u1363 \u12a0\u1295\u12f3\u1295\u12f5 \u1241\u122d\u1325\u122b\u132e\u127d \u1245\u122d\u1265 \u1260\u1206\u1291 \u1241\u122d\u1325\u122b\u132e\u127d \u120a\u1273\u1308\u12f5 \u12ed\u127d\u120b\u120d \u1361\u1361) \u12f6 \/ \u122d \u1233\u126d\u1275\u1279\u12ad \u1201\u1208\u1275 \u12b3\u1236\u127d\u1295\u1293 \u1328\u1228\u122d \u12a5\u12ed\u1273\u12ce\u127d\u1295 \u1260\u121a\u12eb\u1235\u1308\u129d \u12a5\u1293 \u12d3\u12ed\u1290 \u1235\u12cd\u122d \u1266\u1273\u12ce\u127d\u1295 \u1260\u12a8\u134d\u1270\u129b \u1201\u1294\u1273 \u1260\u1218\u1240\u1290\u1235 \u12ed\u1205\u1295\u1295 \u127d\u130d\u122d \u12ed\u1348\u1273\u120d \u1361\u1361 \u12a5\u1231 \u12f0\u130d\u121e \u12e8\u1215\u130d \u1218\u1235\u1218\u122e\u1279\u1295 \u12e8\u130a\u12dc \u1245\u12f0\u121d \u1270\u12a8\u1270\u120d \u12a5\u12e8\u1270\u12a8\u1270\u1208 \u1290\u12cd - \u1260\u134d\u1325\u1290\u1275 \u12e8\u121a\u12eb\u1290\u1243\u1243 - \u1235\u1208\u12da\u1205 \u1270\u1218\u122b\u121b\u122a\u12ce\u127d \u12e8\u1275\u129b\u12cd\u1295 \u12a5\u1295\u1245\u1235\u1243\u1234 \u12e8\u1275\u129b\u12cd \u1263\u1208 -3-\u120d \u1236\u1235\u1275-\u120d\u12ac\u1275 \u121e\u12f4\u120d \u1208\u1218\u1308\u1295\u1263\u1275 \u12a0\u1235\u1348\u120b\u130a \u12a5\u1295\u12f0\u1206\u1290 \u12eb\u12cd\u1243\u1209\u1362<\/p>\n \u12e8\u12f6 \/ \u122d \u1233v\u1275\u1279\u12ad \u1355\u122e\u1300\u12ad\u1275 \u1260\u1218\u1300\u1218\u122a\u12eb \u1235\u122d\u12d3\u1271\u1295 \u1260\u12ae\u121d\u1352\u12e9\u1270\u122d \u121b\u1245\u1228\u1262\u12eb\u12ce\u127d \u12f2\u12db\u12ed\u1295 \u121b\u12f5\u1228\u130d \u12a5\u1293 \u12a8\u12da\u12eb \u1218\u1270\u130d\u1260\u122a\u12eb\u12cd\u1295 \u1260\u1218\u12f3\u134a\u1275 \u121e\u12f4\u120e\u127d \u121b\u1228\u130b\u1308\u1325\u1295 \u12eb\u12ab\u1275\u1273\u120d \u1361\u1361 \u12e8\u12a5\u1231 \u130d\u1265 \u1260\u1328\u1228\u122d \u1328\u1228\u122d \u1260\u1218\u1328\u1218\u122d \u12a5\u1295\u12f2\u1201\u121d \u12c8\u12f0 \u1203\u122d\u12f5\u12cc\u122d \u12a5\u1293 \u1236\u134d\u1275\u12cc\u122e\u127d \u1260\u121a\u12f0\u1228\u1309 \u121b\u123b\u123b\u12eb\u12ce\u127d \u12a0\u121b\u12ab\u12ed\u1290\u1275 \u12a0\u1201\u1295 \u120b\u1208\u1201\u1208\u1275 \u134e\u1295\u1276\u1295 \u121b\u12ed\u12ad\u122e-\u134b\u12ed\u120e\u127d\u1295 \u1208\u121b\u12d8\u1218\u1295 \u12e8\u121a\u12eb\u1235\u127d\u1209 \u1218\u1295\u1308\u12f6\u127d\u1295 \u121b\u12d8\u130b\u1300\u1275 \u1290\u12cd \u1361\u1361<\/p>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>2021-2022<\/h3>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>
2020-2021<\/h3>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>
2019-2020<\/h3>\n\n\t\t<\/div>\n\t<\/div>\n<\/div><\/div><\/div><\/div>